Elevated interleukin-33, CD1c+ dendritic cells, and interleukin-17A in peritoneal fluid of patients with endometriosis: a case-control study.

Wen Yuan, Jiao Huang, Tao He, Huanni Li, Xianqing Wu

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Wen YuanDepartment of Obstetrics and Gynecology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, 410004, China.

Jiao HuangDepartment of Obstetrics and Gynecology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, 410004, China.

Tao HeDepartment of Obstetrics and Gynecology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, 410004, China.

Huanni LiDepartment of Obstetrics and Gynecology, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, 410004, China.

Xianqing WuDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital of Central South University, 139 Middle Renming Road, Changsha, 430011, China. 13574838303@163.com.

Funding

Fundamental Research Funds for the Central Universities of Central South University 2021zzts1061National Natural Science Foundation of China 81873826

6 · The paper itself

Abstract

backgroundEndometriosis (EMS) is a chronic inflammatory disorder involving ectopic endometrial tissue growth. This study investigated IL-33, CD1c+ dendritic cells (DCs) and their co-stimulatory molecules (CD40, CD80, CD86), and IL-17 A in the peritoneal fluid (PF) of EMS patients, and explored their interrelationships. METHODS AND

resultsPF samples were obtained from 42 women with EMS as experimental subjects and 31 women without EMS as controls. Enzyme-linked immunosorbent assay (ELISA) showed that PF IL-33 (P < 0.001) and IL-17 A (P < 0.01) levels were significantly elevated in EMS patients vs. controls. Moreover, IL-33 levels were significantly higher in stages III-IV than I-II (P < 0.01). Flow cytometry (FCM) revealed no significant difference in the proportion of CD1c + DCs within EMS patient PF compared to controls (P > 0.05). However, the proportions of CD1c+CD40+ (P < 0.001), CD1c+CD80+ (P < 0.05), and CD1c+CD86+ (P < 0.01) DCs were significantly elevated in EMS, with CD1c+CD40 + higher in stages III-IV vs. I-II (P < 0.05). For in vitro experiments, control PF mononuclear cells (PFMCs) were incubated with recombinant IL-33 (100 ng/mL) for 24 h. IL-33 treatment increased IL-17 A production and CD1c+CD40 + and CD1c+CD80 + DC frequences in control PFMCs compared to unstimulated PFMCs (all P < 0.05).

conclusionElevated IL-33 and IL-17 A levels, along with enhanced CD1c + DC maturation and activation in PF, may contribute to EMS pathogenesis and progression. IL-33 may promote disease development by inducing CD1c + DC maturation/activation, thus increasing IL-17 A production.

Indexed as

Antigens, CD1Ascitic FluidDendritic CellsEndometriosisInterleukin-17Interleukin-33AdultCase-Control StudiesFemaleGlycoproteinsHumansAntigens, CD1CD1C protein, humanGlycoproteinsIL17A protein, humanIL33 protein, humanInterleukin-17Interleukin-33CD1c+Dendritic cellsEndometriosisIL-17AIL-33Peritoneal fluid

Identifiers

PMID42593546

DOI10.1016/j.cellimm.2019.02.003

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