Evidence map›Paper›PMID 42592681›Full record

ReviewBioEssays : news and reviews in molecular, cellular and developmental biology2026

The Single-Stranded DNA Platform: A Potential Broad-Spectrum Vector for Editing Genes in Prokaryotes: ssDNA May Break Through the Difficulties of Genetic Manipulation on Non-Model Bacteria.

Huayi Wang, Yefeng Wang, Dingkun Chang, Jie Fang, Zixuan Zheng, Xiangfeng Wang, Hongju Yang, Zhi Liu

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in BioEssays : news and reviews in molecular, cellular and developmental biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Huayi WangKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Yefeng WangKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Dingkun ChangKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Jie FangKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Zixuan ZhengKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Xiangfeng WangKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Hongju YangDivision of Geriatric Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
Zhi LiuKey Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Funding

Major Science and Technology Special Plan of Yunnan Province 202402AA310011National Key R&D Program of China 2024YFA0918500Natural Science Foundation of Huibei Province 2025AFD249
6 · The paper itself

Abstract

Single-stranded DNA (ssDNA) is an emerging nucleic acid tool with the potential to become a more efficient genetic delivery vector than traditional plasmids in prokaryotes. While conventional double-stranded DNA (dsDNA) vectors are easily recognized and attacked by bacterial defense systems, ssDNA's structural differences make it resistant to corresponding enzymatic recognition. Moreover, the presence of unpaired bases in ssDNA enables efficient binding with other molecules, allowing for regulated gene expression and targeted delivery of other nucleic acids or molecules. In this study, we investigated the natural forms of ssDNA and summarized its synthesis and introduction methods. By analyzing existing applications of ssDNA in eukaryotes, we demonstrated the strategies and advantages of applying it to genetic manipulation in prokaryotes.

Indexed as

BacteriaDNA, Single-StrandedGene EditingGenetic VectorsProkaryotic CellsDNA, Single-Strandedbacterial defense systemgene editinggenetic vectormethylationsingle‐strand DNA

Identifiers

PMID42592681
PMCPMC13470472

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.