ArticleArteriosclerosis, thrombosis, and vascular biology2026
Single-Cell Proteomics Uncovers Cell-Specific Proteins in Vascular Health and Marfan Syndrome Disease-Brief Report.
Junedh M Amrute, Lihua Jiang, Nikhita Bolar, Kelly Higa, Chenchen Zhu, Ruiqi Jian, Jennifer Kim, Matthew Duda, Anna Marie Puaala, Avani Klinder and 6 more
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In one paragraphArticle in Arteriosclerosis, thrombosis, and vascular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
16 authors.
Junedh M Amrute *Center for Cardiovascular Research, Division of Cardiology, Department of Medicine, Washington University School of Medicine, St. Louis, MO (J.M.A.).ORCID 0000-0002-6851-0168 Lihua Jiang *Department of Genetics (L.J., C.Z., R.J., M.P.S.), Stanford University School of Medicine, CA.ORCID 0000-0002-8442-8674 Nikhita BolarDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.
Kelly HigaDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0001-8409-9017 Chenchen ZhuDepartment of Genetics (L.J., C.Z., R.J., M.P.S.), Stanford University School of Medicine, CA.
Ruiqi JianDepartment of Genetics (L.J., C.Z., R.J., M.P.S.), Stanford University School of Medicine, CA.
Jennifer KimDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0002-2313-2685 Matthew DudaDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0003-1442-7614 Anna Marie PuaalaDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.
Avani KlinderDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.
Alex DalalDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0002-9993-3817 Albert J PedrozaDepartment of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0001-5291-5980 Dieter P ReinhardtDepartment of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada (D.P.R.).ORCID 0000-0001-6535-9872 Michael P Snyder *Department of Genetics (L.J., C.Z., R.J., M.P.S.), Stanford University School of Medicine, CA.ORCID 0000-0003-0784-7987 Michael P Fischbein *Department of Cardiothoracic Surgery (N.B., K.H., J.K., M.D., A.M.P., A.K., A.D., A.J.P., M.P.F.), Stanford University School of Medicine, CA.ORCID 0000-0002-0638-2025 Funding
Marfan Aortic Embryologic Origin Influences Aneurysm FormationR01HL157949 · NHLBI · STANFORD UNIVERSITY · PI FISCHBEIN, MICHAEL PETER · 2022 to 2025
$2.6MMultiparametric deep tissue microscope for in vivo and in vitro imagingS10OD032300 · OD · STANFORD UNIVERSITY · PI O'BRIEN, LUCY ERIN · 2022 to 2022
$600kNHLBI NIH HHS R01 HL157949NIH HHS S10 OD032300
6 · The paper itselfAbstract
backgroundThe vascular system is the largest organ in the body and underlies most chronic diseases, yet the molecular mechanisms that govern its plasticity remain poorly defined.
methodsWe applied single-cell proteomics in vascular disease, integrating it with single-cell transcriptomics to map protein regulation in healthy and Marfan syndrome aortas.
resultsThis approach uncovered cell type-specific and cell state-specific proteins missed at the transcriptional level. Notably, we identified a decoupling of fibrillin-1 RNA and protein abundance, suggesting altered protein regulation as a potential contributor to aortic degeneration. Single-cell proteomics further resolved modulated smooth muscle cell states enriched for matrix effectors (AEBP1 [adipocyte enhancer binding protein 1], HTRA1 [high-temperature requirement A serine peptidase 1], FN1 [fibronectin 1]) and uniquely protein-level regulators (GLIPR2 [GLI pathogenesis-related 2], ITGB2 [integrin beta-2], and CD151 [cluster of differentiation 151 antigen]).
conclusionsTogether, these findings establish the human single-cell proteomic atlas of the aorta, reveal altered FBN1 (fibrillin-1) protein regulation in both human and mouse Marfan syndrome, and position single-cell proteomics as a transformative framework for decoding vascular plasticity and identifying actionable effectors in disease.
Indexed as
AortaMarfan SyndromeMuscle, Smooth, VascularMyocytes, Smooth MuscleProteomicsSingle-Cell AnalysisAdipokinesAnimalsCase-Control StudiesDisease Models, AnimalFibrillin-1HumansMaleMiceSingle-Cell Gene Expression AnalysisAdipokinesFBN1 protein, humanFbn1 protein, mouseFibrillin-1aneurysmfibrillin-1Marfan syndromeproteomicssmooth muscle
Identifiers
PMID42592649
PMCPMC13474995
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