Evidence map›Paper›PMID 42591798›Full record

ArticleIBRO neuroscience reports2026

The ketogenic diet ameliorates behavioral abnormalities in a PTZ-induced zebrafish larvae model via modulating neurotransmitter balance.

Xue Yang, Jia Lin, Qi Zhang, Yuheng Xia, Shuizhen Zhou, Yi Wang, Qiang Li, Lifei Yu

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In one paragraph

Article in IBRO neuroscience reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xue YangDepartment of Neurology, National Children's Medical Center, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Jia LinTranslational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect Prevention and Control, NHC Key Laboratory of Neonatal Diseases, Institute of Pediatrics, Children's Hospital of Fudan University, National Children's Medical Center, 210013, Shanghai, China.
Qi ZhangTranslational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect Prevention and Control, NHC Key Laboratory of Neonatal Diseases, Institute of Pediatrics, Children's Hospital of Fudan University, National Children's Medical Center, 210013, Shanghai, China.
Yuheng XiaShanghai World Foreign Language Academy, No. 400 Baihua Street, XuhuiDistrict, China.
Shuizhen ZhouDepartment of Neurology, National Children's Medical Center, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Yi WangDepartment of Neurology, National Children's Medical Center, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Qiang LiTranslational Medical Center for Development and Disease, Shanghai Key Laboratory of Birth Defect Prevention and Control, NHC Key Laboratory of Neonatal Diseases, Institute of Pediatrics, Children's Hospital of Fudan University, National Children's Medical Center, 210013, Shanghai, China.
Lifei YuDepartment of Neurology, National Children's Medical Center, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: The ketogenic diet (KD) is a well-established therapy for drug-resistant epilepsy, reducing seizure burden in children and improving cognition in epileptic encephalopathy patients, but its underlying mechanisms remain incompletely understood. This study aimed to establish a translational zebrafish model to investigate KD's anti-epileptic potential and mechanisms, focusing on neurotransmitter homeostasis. Method: Acute epilepsy was induced in 7-day post-fertilization (dpf) zebrafish larvae (n = 24/group, randomly assigned) using pentylenetetrazol (PTZ), a selective GABA-A receptor antagonist. KD was administered to the treatment group, with behavioral assays (locomotor activity, thigmotaxis), electroencephalographic (EEG) recordings, and high-performance liquid chromatography (HPLC) for neurotransmitter quantification. Statistics were analyzed via two-way ANOVA/Tukey's test or Student's t-test. Results: Results showed KD significantly ameliorated PTZ-induced epileptiform behaviors (prolonged seizure latency, reduced stage-specific duration), attenuated EEG multi-peak discharges, increased inhibitory neurotransmitters (GABA, glycine, taurine), and reversed PTZ-induced excitatory-inhibitory neurotransmitter imbalance (e.g., Glu/GABA, Gln/Tau ratios). Conclusion: We established a PTZ-induced amphibian epilepsy model with KD intervention. KD exerts anti-epileptic effects, likely mediated partially by rebalancing central nervous system neurotransmitter homeostasis, providing a valuable translational platform for further mechanism research.

Indexed as

Ketogenic diet treatmentNeurotransmitterPentylenetetrazolZebrafish

Identifiers

PMID42591798
PMCPMC13463253

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.