Evidence map›Paper›PMID 42591369›Full record

ArticleBio-protocol2026

Mouse and Rat Oxygen-Induced Retinopathy Models to Study Vascular Features Seen in Retinopathy of Prematurity.

Morgan Paige Tankersley, Shreya Beri, Aniket Ramshekar, Bright Asare-Bediako, Neal Sandeep Shah, James Regun Karmoker, Heng-Chiao Huang, Elizabeth M Hartnett

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Morgan Paige TankersleyByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Shreya BeriByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Aniket RamshekarByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Bright Asare-BediakoByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Neal Sandeep ShahByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
James Regun KarmokerByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Heng-Chiao HuangByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.
Elizabeth M HartnettByers Eye Institute, Department of Ophthalmology, Stanford University School of Medicine, Stanford, CA, USA.

Funding

Stanford Vision Research CoreP30EY026877 · NEI · STANFORD UNIVERSITY · PI Jeffrey L Goldberg · 2017 to 2026
$8.0M
Mechanisms of Angiogenesis of Retinopathy of PrematurityR01EY015130 · NEI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Mary Elizabeth Ruth Hartnett · 2005 to 2026
$7.6M
Stanford Ophthalmology Advanced Research ProgramR38EY037090 · NEI · STANFORD UNIVERSITY · PI Yang Sun · 2025 to 2026
$495k
NEI NIH HHS P30 EY026877NEI NIH HHS R01 EY015130NEI NIH HHS R38 EY037090
6 · The paper itself

Abstract

Retinopathy of prematurity (ROP), a retinovascular disease, is a leading cause of childhood blindness worldwide. Given the constraints of studying molecular mechanisms in preterm infants, reproducible animal models are important to understand ROP pathophysiology. Mouse and rat oxygen-induced retinopathy (OIR) models are the most commonly used and recapitulate key vascular features seen in ROP. However, these models are susceptible to inherent variability that limits reproducibility, including inter-litter variability, consistency of oxygen delivery across experiments, retinal dissection technique, and immunohistochemistry. Here, we describe a comprehensive protocol for performing the most common mouse and rat OIR models, and procedures such as eye enucleation, retinal dissection and flat mounting, isolectin GS-IB4 staining, whole retina stitched fluorescence imaging from Z-stacks, and quantification of vascular features. This protocol provides important materials and procedural details to increase the reproducibility of the mouse and rat OIR models. Key features • Rat and mouse oxygen-induced retinopathy (OIR) models. • Eye enucleation of experimental rat and mouse pups. • Retinal flat mounting and immunostaining for rat and mouse eyes. • Image analysis of retinal flat mounts from rat and mouse eyes.

Indexed as

Lectin immunostainingOxygen-induced retinopathyRetinal dissectionRetinal flat mountingRetinopathy of prematurityVascular biology

Identifiers

PMID42591369
PMCPMC13461755

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.