Evidence map›Paper›PMID 42590857›Full record

ArticleAdvanced healthcare materials2026

Hyperbranched Polylysine-Based Lipopolyplexes Enable Efficient mRNA Delivery With Long-Term Storage Stability.

Huidi Meng, Bingjie Fu, Min Liang, Qiaoxuan Wang, Xingpu Liu, Man Li, Qiaoju Hu, Yiyuan Duan, Changyou Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Huidi MengCollege of Engineers, Zhejiang University, Hangzhou, China.ORCID https://orcid.org/0009-0001-1289-2208
Bingjie FuCollege of Engineers, Zhejiang University, Hangzhou, China.
Min LiangZhejiang Engineering Research Center for Interface Technology of Medical Polymers and Devices, Shaoxing Key Laboratory of Healthcare Materials and Application Technology, and Center for Healthcare Materials, Shaoxing Institute, Zhejiang University, Shaoxing, China.
Qiaoxuan WangZhejiang Key Laboratory of Advanced Organic Materials and Technologies, MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
Xingpu LiuCollege of Engineers, Zhejiang University, Hangzhou, China.
Man LiCollege of Engineers, Zhejiang University, Hangzhou, China.
Qiaoju HuZhejiang Engineering Research Center for Interface Technology of Medical Polymers and Devices, Shaoxing Key Laboratory of Healthcare Materials and Application Technology, and Center for Healthcare Materials, Shaoxing Institute, Zhejiang University, Shaoxing, China.
Yiyuan DuanZhejiang Engineering Research Center for Interface Technology of Medical Polymers and Devices, Shaoxing Key Laboratory of Healthcare Materials and Application Technology, and Center for Healthcare Materials, Shaoxing Institute, Zhejiang University, Shaoxing, China.
Changyou GaoCollege of Engineers, Zhejiang University, Hangzhou, China.ORCID https://orcid.org/0000-0001-5084-7208

Funding

111 Project B16042Leading Innovative and Entrepreneurial Team of Zhejiang Province 2024R02013National Key Research and Development Program of China 2023YFE0108700National Natural Science Foundation of China U22A20155State Key Laboratory of Transvascular Implantation Devices 012024004
6 · The paper itself

Abstract

Clinical translation of messenger RNA (mRNA) therapeutics is often hampered by the poor storage stability of lipid-based delivery vectors. Many lipopolyplex (LPP) delivery systems struggle to achieve efficient nucleic acid release through the membrane fusion pathway. Herein, we developed a novel ternary lipopolyplex (LPP) platform of lipid/HBPL/mRNA LPPs by incorporating hyperbranched poly-L-lysine (HBPL) as a functional polymeric core. The highly branched architecture of HBPL enabled a unique balance between high mRNA encapsulation efficiency, efficient intracellular release, and exceptional colloidal stability, thereby overcoming a key limitation of conventional lipid nanoparticles. This platform primarily promoted cellular uptake through a membrane fusion mechanism in vitro, thereby achieving highly efficient cytoplasmic delivery, while the HBPL core enabled superior mRNA release compared to its linear ε-Polylysine (ε-PLL, hereafter referred to as PLL)-based counterpart. These attributes collectively yielded high transfection efficiency across multiple cell lines, significantly enhanced dendritic cell (DC) maturation, and superior biocompatibility over a commercial transfection reagent. Efficient mRNA delivery to the lungs and spleen was also confirmed in vivo, with protein expression sustained even after 1 month of storage. By successfully integrating long-term stability with high delivery efficiency, this HBPL-based LPP platform represents a highly promising candidate for advancing mRNA therapeutics and vaccines.

Indexed as

LipidsPolylysineRNA, MessengerAnimalsDendritic CellsHumansMiceNanoparticlesTransfectionLipidsPolylysineRNA, Messengerhyperbranched polylysinelipopolyplexesmembrane fusionmRNA deliverystability

Identifiers

PMID42590857
PMCPMC13568904

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.