Evidence map›Paper›PMID 42589208›Full record

ArticleInternational journal of molecular sciences2026

Primer-Less Species Identification Throughout Fungal (

Tadeusz Malewski, Slavica Matić, Aleksandra Gabriela Bilska, Maria Alexandra Cucu, Laura Miozzi, Antonietta Mello, Andrzej Skwiercz, Tomasz Oszako, Justyna Anna Nowakowska

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tadeusz MalewskiDepartment of Molecular Evolution, Museum and Institute of Zoology, Polish Academy of Sciences, 00-818 Warsaw, Poland.ORCID 0000-0001-8061-435X
Slavica MatićInstitute for Sustainable Plant Protection (IPSP), National Research Council of Italy (CNR), Strada delle Cacce 73, 10135 Turin, Italy.ORCID 0000-0002-0885-8744
Aleksandra Gabriela BilskaDepartment of Molecular Evolution, Museum and Institute of Zoology, Polish Academy of Sciences, 00-818 Warsaw, Poland.ORCID 0000-0002-2047-0697
Maria Alexandra CucuInstitute for Sustainable Plant Protection (IPSP), National Research Council of Italy (CNR), Strada delle Cacce 73, 10135 Turin, Italy.ORCID 0000-0002-6113-9458
Laura MiozziInstitute for Sustainable Plant Protection (IPSP), National Research Council of Italy (CNR), Strada delle Cacce 73, 10135 Turin, Italy.
Antonietta MelloInstitute for Sustainable Plant Protection (IPSP), National Research Council of Italy (CNR), Viale Pier Andrea Mattioli 25, 10125 Turin, Italy.ORCID 0000-0002-6311-377X
Andrzej SkwierczThe National Institute of Horticultural Research, 96-100 Skierniewice, Poland.ORCID 0000-0002-7008-5674
Tomasz OszakoForest Protection Department, Forest Research Institute, 05-090 Raszyn, Poland.ORCID 0000-0003-4688-2582
Justyna Anna NowakowskaFaculty of Biology and Environmental Sciences, Institute of Biological Sciences, Cardinal Stefan Wyszyński University in Warsaw, 01-938 Warsaw, Poland.ORCID 0000-0001-5863-053X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate species identification is essential for biodiversity studies, ecological monitoring, and biosecurity, but current molecular approaches rely on PCR amplification, which requires universal primers. Here, we evaluated the possibility of primer-free species identification via direct RNA sequencing (dRNA-seq) by using Oxford Nanopore Technology (ONT). Total RNA isolated from three major eukaryotic kingdoms-fungal (

Indexed as

FungiSequence Analysis, RNAAnimalsPhylogenybiodiversity assessmentdirect RNA sequencingprimer-free barcodingspecies identification

Identifiers

PMID42589208
PMCPMC13465249

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.