Ginsenoside Rc Improves Chilled Storage Quality of Lower-Layer X-Bearing Dairy Goat Spermatozoa Through Enhanced Mitochondrial Function and Antioxidant Capacity.

Songmao Guo, Xing Zhang, Xu Zhang, Zitong Hu, Yu Li, Fei Wen, Ming Xian, Zhangtao Hu, Jianhong Hu

Abstract read

In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Songmao GuoKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Xing ZhangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Xu ZhangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Zitong HuKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Yu LiKey Laboratory for Efficient Ruminant Breeding Technology of Higher Education Institutions in Shaanxi Province, Shaanxi A&F Technology University, Yangling 712100, China.

Fei WenKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Ming XianKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Zhangtao HuKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.

Jianhong HuKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.ORCID 0000-0002-5833-3444

Funding

National Key Research and Development Program project 2022YFD1300200Shaanxi Provincial Key Research and Development Program 2024NC-ZDCYL-03-02

6 · The paper itself

Abstract

Sex-control technologies that alter offspring sex ratios can markedly improve the economic efficiency of dairy production. Recent studies have demonstrated that treatment with the TLR7/8 agonist R848 enables successful in vitro separation of X- and Y-bearing spermatozoa in mice, cattle, and goats. This is because high-quality semen is essential for successful artificial insemination, especially during chilled storage. In this study, X/Y sperm-specific activation and sorting methods were used to isolate sperm from dairy goats, and the effectiveness of sperm isolation was evaluated using TLR7 immunofluorescence staining. Different concentrations of ginsenoside Rc were added to the semen diluent as a natural preservative, and the lower-layer X-bearing sperm recovered from the sorting system were subjected to chilled storage. The effects of ginsenoside Rc on chilled storage performance were then assessed. Among the tested concentrations, 150 μg/mL of ginsenoside Rc exhibited the most potent protective effect, significantly improving the quality, mitochondrial function, antioxidant capacity and the relative protein abundance of SIRT1, PGC-1α, and Nrf1 of the lower-layer X-bearing sperm after chilled storage for 7 days. In summary, supplementation with ginsenoside Rc in the chilled extender improved the quality of lower-layer X-bearing dairy goat spermatozoa during chilled storage by maintaining mitochondrial function, enhancing antioxidant defense, and reducing oxidative damage, facilitating the application of lower-layer X-bearing sperm-selected sperm in production.

Indexed as

chilled storagedairy goat spermginsenoside Rcreactive oxygen speciestoll-like receptor 7/8

Identifiers

PMID42589165

DOI10.3390/biology15151298

PMCPMC13465667

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.