miR-Novel-80 Suppresses Porcine Reproductive and Respiratory Syndrome Virus Replication by Targeting the Viral

Shuo Feng, Yiwen Pei, Xue Gao, Danjiao Yang, Jie Liu, Zijing Guo, Zhidong Zhang, Long Zhou

Abstract read

In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuo FengKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.

Yiwen PeiKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.ORCID 0009-0001-2849-7296

Xue GaoKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.

Danjiao YangInstitute of Animal Science of Ganzi Tibetan Autonomous Prefecture of Sichuan Province, Kangding 626000, China.

Jie LiuKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.

Zijing GuoKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.

Zhidong ZhangKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.ORCID 0000-0002-2186-7661

Long ZhouKey Laboratory of Animal Medicine of Sichuan Education Department, College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China.

Funding

Ganzi Prefecture Science and Technology Plan Project 25kjjh0013National Key Research and Development Program Project 2024YFD1800503Sichuan Science and Technology Program 2025ZNSFSC0192

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is a major infectious disease that poses a severe threat to the global swine industry. To investigate the role of miRNAs in the infection and susceptibility of PRRSV, four miRNA libraries were constructed and sequenced from PRRSV-infected and mock-infected of Tibetan pigs and Large White pigs at 7 days post-infection. A novel miRNA, miR-novel-80, was differentially expressed between PRRSV-infected and mock-infected porcine alveolar macrophages from 2 pig breeds. Importantly, the over-expression of miR-novel-80 inhibited the replication of a PRRSV-1 strain and multiple lineages (L1, L5, and L8) of PRRSV-2 strains in a dose-dependent manner. Bioinformatic predictions and experimental validation demonstrated that miR-novel-80 restricts viral replication through a dual antiviral mechanism. Directly, it targets the PRRSV

Indexed as

CXXC finger protein 4 (CXXC4)microRNAnsp1porcine reproductive and respiratory syndrome virus (PRRSV)

Identifiers

PMID42589072

DOI10.3390/ani16152434

PMCPMC13464968

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.