ArticleAnimals : an open access journal from MDPI2026
Effects of HMG on Superovulation Efficacy in Kazakh Sheep and Screening of Plasma Reproductive Hormones and Metabolic Markers.
Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Superovulation and embryo transfer techniques are effective strategies for improving reproductive efficiency in sheep flocks. To characterize changes in plasma metabolites and reproductive hormones following HMG or FSH superovulatory treatment, and to screen for biomarkers closely associated with embryo quality, we employed liquid chromatography-based untargeted metabolomics and enzyme-linked immunosorbent assay (ELISA) to analyze plasma samples from donor ewes on day 6 after hormone administration. This study aimed to identify small-molecule biomarkers affecting embryo quality under different hormonal regimens. A total of 46 Kazakh ewes were allocated into two groups: the HMG group received HMG treatment, while the FSH group received FSH treatment; both groups were superovulated using a tapering dose regimen administered via intramuscular injection. Our results show that the average number of viable embryos in the HMG group (5.36 ± 3.29,
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