Substrate Concentration Modulates Peptide Profile and Bioactivity in Alcalase Hydrolysis of Bovine Plasma Proteins at Fixed Enzyme-to-Substrate Ratio.

Omar A Figueroa-Moreno, José E Zapata-Montoya, Ismael Marcet, Manuel Rendueles, Natalia Díaz Fernandez

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Omar A Figueroa-MorenoFaculty of Engineering, University of La Guajira, Riohacha 440002, Colombia.ORCID 0000-0001-9859-9661

José E Zapata-MontoyaDepartment of Food, Faculty of Pharmaceutical and Food Sciences, University of Antioquia, Carrera 51, Number 62-13, Medellin 050010, Colombia.ORCID 0000-0003-2733-1515

Ismael MarcetDepartment of Chemical and Environmental Engineering, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain.ORCID 0000-0003-0949-3684

Manuel RenduelesDepartment of Chemical and Environmental Engineering, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain.ORCID 0000-0001-7056-1231

Natalia Díaz FernandezDepartment of Physical and Analytical Chemistry, University of Oviedo, C/Julián Clavería 8, 33006 Oviedo, Spain.

Funding

University of La Guajira institutional academic support

6 · The paper itself

Abstract

Many studies have been conducted testing different enzymes and protein combinations to produce peptides with different types and levels of bioactivities; however, their effect on peptide sequence, peptide size distribution, and bioactivity, while maintaining a constant enzyme-substrate ratio, has been less frequently addressed. In this research, bovine plasma has been hydrolysed by Alcalase at several substrate concentrations (5, 10, 15, and 20 g/L) and at a fixed enzyme-substrate ratio of 1:25 (

Indexed as

Blood ProteinsPeptidesSubtilisinsAngiotensin-Converting Enzyme InhibitorsAnimalsAntihypertensive AgentsAntioxidantsBioactive Peptides, DietaryCattleChromatography, High Pressure LiquidHydrolysisMolecular WeightSubstrate SpecificityTandem Mass SpectrometryAngiotensin-Converting Enzyme InhibitorsAntihypertensive AgentsAntioxidantsBioactive Peptides, DietaryBlood ProteinsPeptidesSubtilisinsAlcalaseantihypertensiveantioxidantbovine plasmahydrolysissubstrate concentration

Identifiers

PMID42588541

DOI10.3390/molecules31152693

PMCPMC13467922

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.