Evidence map›Paper›PMID 42587753›Full record

ArticleCells2026

Profiling miRNAs Involved in Human Oligodendrocyte Precursor Cell Differentiation and Maturation.

Mansoureh Barzegar, Asmita Dhukhwa, Vaidehi Nilesh Patel, Fernanda C Velasquez, Samarjit Das, Arun H Patil, Marc K Halushka, Xitiz Chamling

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Mansoureh BarzegarDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.ORCID 0000-0003-2747-9974
Asmita DhukhwaDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Vaidehi Nilesh PatelDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.ORCID 0000-0001-5340-8681
Fernanda C VelasquezDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Samarjit DasDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.ORCID 0000-0002-4377-2823
Arun H PatilLieber Institute for Brain Development, The Johns Hopkins University, Baltimore, MD 21218, USA.ORCID 0000-0002-3125-8958
Marc K HalushkaPathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH 44195, USA.ORCID 0000-0002-7112-7389
Xitiz ChamlingDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.ORCID 0000-0001-6401-7715

Funding

Developing Remyelination Strategies for Demyelinating Optic Neuropathies Using Human Pluripotent Stem CellsR00EY029011 · NEI · JOHNS HOPKINS UNIVERSITY · PI CHAMLING, XITIZ · 2022 to 2024
$731k
American Heart Association 22TPA 970850 and 26BTPA1622826Early Investigator Award from the DOD-MSRP- HT94252510513Maryland Stem Cell Research Fund 2023-MSCRFF-6132Myelin Repair Foundation NANEI NIH HHS R00 EY029011NEI NIH HHS R00EY029011Research to Prevent Blindness NAThe Gilbert Family Foundation NAUnited States Department of Defense HT94252510513
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are conserved post-transcriptional regulators that play essential roles in cellular development and differentiation. Although miRNAs involved in oligodendrocyte lineage cell (OLLC) differentiation have been extensively studied in rodent models, their functions in human oligodendrocyte (OL) development remain poorly understood. To address this, we used a human embryonic stem cell (hESC) reporter system and an optimized differentiation protocol to generate and isolate well-characterized OLLCs at defined developmental stages. Next-generation sequencing-based miRNA profiling identified stage-specific miRNAs associated with OL lineage specification and maturation. In addition to canonical OL-associated miRNAs, we identified several previously uncharacterized oligodendrocyte progenitor cell (OPC)/oligodendrocyte (OL)-enriched miRNAs, including miR-3943, miR-4286, miR-1296-5p, miR-488-3p, miR-675-5p, and miR-128-3p, as potential novel regulators and molecular markers for human OLLC development. Computational target analysis further identified candidate regulatory genes, including ZNF488, DLX1, CSNK2B, and KCNJ1, and predicted their association with AKT, SMAD2/3, estrogen-receptor, and insulin-signaling pathways. Together, these findings provide a comprehensive stage-specific miRNA resource for human OL lineage development and provide insight into potential miRNA-mediated regulatory networks governing human OL development.

Indexed as

Cell DifferentiationGene Expression ProfilingMicroRNAsOligodendrocyte Precursor CellsOligodendrogliaCell LineageHuman Embryonic Stem CellsHumansMicroRNAshOPChPSCshuman oligodendrocyte differentiationhuman oligodendrocyte maturationhuman oligodendrocyte progenitor cellshuman oligodendrocyteshuman pluripotent stem cellsmicroRNA profilingmicroRNAsnext generation sequencing

Identifiers

PMID42587753
PMCPMC13464101

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.