Evidence map›Paper›PMID 42587008›Full record

ArticleScientific reports2026

Cytotoxic profiling of Hedera pastuchovii in human cancer cell lines and its genoprotective effects on normal lymphocytes.

Emran Habibi, Mohammad Shokrzadeh, Omid Abed Khojasteh, Hashem Gerey, Mohammad Hossein Hosseinzadeh, Satyajit D Sarker, Lutfun Nahar

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Emran HabibiCentre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK. E.habibi@ljmu.ac.uk.
Mohammad ShokrzadehDepartment of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Omid Abed KhojastehDepartment of Clinical Pharmacy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Hashem GereyMedicinal Plants Research Centre, Institute of Herbal Medicines and Metabolic Disorders, Mazandaran University of Medical Sciences, Sari, Iran.
Mohammad Hossein HosseinzadehPharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran. mh.hosseinzadeh1997@gmail.com.
Satyajit D SarkerCentre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK.
Lutfun NaharCentre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK. profnahar@outlook.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genus Hedera L. has long been recognized as a valuable source of anticancer agents, such as triterpenoid saponins. In contrast, Hedera pastuchovii Woronow remains largely unexplored. This study investigates the cytotoxic profiles and genoprotective potential of H. pastuchovii leaf extracts. Leaves were extracted with ethanol and fractionated into n-hexane (Hex-F), ethyl acetate (Eth-F), and methanolic (Met-F) fractions using vacuum liquid chromatography. Phenolic, flavonoid, and saponin contents were quantified. Cytotoxicity was evaluated against four human cancer cell lines: A549 (lung), MCF-7 (breast), HeLa (cervical), and SKOV3 (ovarian), using the MTT assay. Apoptosis was assessed by Annexin V/PI flow cytometry, and genotoxicity or genoprotective effects were measured by the micronucleus assay in human lymphocytes. Phytochemical analysis revealed a total saponin yield of 29.95%. The Met-F fraction exhibited the highest concentrations of total phenolics (77.93 mg GAE/g) and triterpenoids (138.62 mg UAE/g), as well as the most pronounced cytotoxic activity, significantly reducing cell viability across all tested cancer lines in a concentration-dependent manner. The IC

Indexed as

Antineoplastic Agents, PhytogenicLymphocytesPlant ExtractsApoptosisCell Line, TumorCell SurvivalHeLa CellsHumansMicronucleus TestsPlant LeavesSaponinsAntineoplastic Agents, PhytogenicPlant ExtractsSaponinsApoptosis inductionCytotoxicityGenoprotective effectHedera pastuchoviiTriterpenoid saponins

Identifiers

PMID42587008
PMCPMC13470190

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