Evidence map›Paper›PMID 42586205›Full record

ArticleCellular and molecular gastroenterology and hepatology2026

Single-Cell and Spatial Transcriptomics Across Populations With Perianal Fistulizing Crohn's Disease Shows Proinflammatory CD4

Sushma C Maddipatla, Sachith Munasinghe, Anne Dodd, Christopher Tastad, Rachel Moss, Hong Yin, Shanta Murthy, Abhishek Dash, Vasantha L Kolachala, David J Cutler and 3 more

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Article in Cellular and molecular gastroenterology and hepatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Sushma C MaddipatlaDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
Sachith MunasingheDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
Anne DoddDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
Christopher TastadDepartment of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.
Rachel MossDepartment of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.
Hong YinDepartment of Pathology, Children's Healthcare of Atlanta, Atlanta, Georgia.
Shanta MurthyDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
Abhishek DashDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
Vasantha L KolachalaDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia.
David J CutlerDepartment of Human Genetics, Emory University School of Medicine, Atlanta, Georgia.
Judy ChoDepartment of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.
Subra KugathasanDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia; Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia; Department of Pediatric Gastroenterology, Children's Healthcare of Atlanta and Pediatric Research Institute, Atlanta, Georgia.
Jason D MatthewsDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia. Electronic address: jdmatt2@emory.edu.

Funding

Gene discoveries in subjects with Crohn's disease of African descentR01DK087694 · NIDDK · EMORY UNIVERSITY · PI KUGATHASAN, SUBRA · 2011 to 2023
$10.3M
Integrative multi-omic risk assessment at diagnosis and during disease progression in African-Americans with Inflammatory bowel diseaseU01DK134191 · NIDDK · EMORY UNIVERSITY · PI SUBRA KUGATHASAN · 2022 to 2026
$2.8M
Genomic Analysis of Perianal Fistulizing Crohn's Disease across AncestriesR01DK125936 · NIDDK · EMORY UNIVERSITY · PI KUGATHASAN, SUBRA · 2020 to 2022
$1.2M
NIDDK NIH HHS R01 DK087694NIDDK NIH HHS R01 DK125936NIDDK NIH HHS U01 DK134191
6 · The paper itself

Abstract

BACKGROUND &

aimsPerianal fistulizing Crohn's disease is a severe morbidity with rectal involvement and a higher prevalence in African Americans compared with European Americans. In this study, we sought to define the distinguishing cellular and molecular features between inflamed rectal Crohn's disease with and without perianal fistula across populations that give new insight into disease severity or progression.

methodsRectal mucosal biopsies were obtained for single-cell sequencing from 50 individuals (262k cells); 18 with Crohn's disease of the rectum with fistula, 25 with Crohn's disease of the rectum without fistula and any other perianal complications, and 7 from individuals with no past or present mucosal disease (controls). Spatial transcriptomics were conducted and analyzed by Sopa and Squidpy, whereas immunofluorescence was processed with QuPath. Patient-derived rectal organoids were used to test inflammatory signaling.

resultsA major distinction between inflamed Crohn's disease of the rectum with and without fistula was the decreased phagocytic signature, antigen processing profile, and unique morphologic structures of proinflammatory CD4

conclusionsDuring inflamed Crohn's disease of the rectum with fistula, CD4

Indexed as

AncestryCD4Crohn's DiseaseMacrophagesMyleoidPerianal FistulaSingle-CellXenium

Identifiers

PMID42586205
PMCPMC13627825

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.