Evidence map›Paper›PMID 42585015›Full record

ArticleSTAR protocols2026

Protocol for optimized methylation context-sensitive enzyme ddRAD library preparation from partially degraded genomic plant DNA.

César Herrero-González, Sylvia Sagen Johnsen, Rómulo Sacramento Sobral, Marian Schubert

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

César Herrero-GonzálezDepartment of Plant Sciences, Norwegian University of Life Sciences (NMBU), Ås 1433, Norway; Natural History Museum, University of Oslo, Research Group Evolution, eDNA, Genomics and Ethnobotany, 0562 Oslo, Norway. Electronic address: c.h.gonzalez@nhm.uio.no.
Sylvia Sagen JohnsenDepartment of Plant Sciences, Norwegian University of Life Sciences (NMBU), Ås 1433, Norway.
Rómulo Sacramento SobralDepartment of Plant Sciences, Norwegian University of Life Sciences (NMBU), Ås 1433, Norway. Electronic address: romulo.sacramento.sobral@nmbu.no.
Marian SchubertDepartment of Plant Sciences, Norwegian University of Life Sciences (NMBU), Ås 1433, Norway. Electronic address: marian.schubert@nmbu.no.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present an optimized methylation context-sensitive enzyme ddRAD (MCSeEd) library-preparation protocol for methylation analysis in CG, CHG, and CHH sequence contexts from plant samples that yield low-quality or partially degraded genomic DNA. We describe steps for quadruple restriction-enzyme digestion and adapter ligation, double size selection, freeze-and-squeeze gel purification, and bead-based cleanup. We then detail a two-step PCR enrichment procedure to generate Illumina-compatible epigenomic libraries.

Indexed as

GeneticsPlant sciencesSequencing

Identifiers

PMID42585015
PMCPMC13487831

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.