Evidence map›Paper›PMID 42584574›Full record

ArticleJournal of the American Chemical Society2026

A Combined Experimental-Theoretical Approach Unveils the Comprehensive Asn Deamidation Kinetics.

Maria Laura De Sciscio, Rosanna Mosetti, Annalisa D'Arco, Stefano Lupi, Mariana Gallo, Thelma A Pertinhez, Fabrizio Vetica, Mauro Giustini, Marco D'Abramo

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Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maria Laura De SciscioDepartment of Chemistry, University of Rome, Sapienza, P.le A. Moro 500185Rome, Italy.
Rosanna MosettiPhysics Department, University of Rome Sapienza, P.le Aldo Moro 500185Rome, Italy.
Annalisa D'ArcoPhysics Department, University of Rome Sapienza, P.le Aldo Moro 500185Rome, Italy.ORCID 0000-0001-7990-5117
Stefano LupiPhysics Department, University of Rome Sapienza, P.le Aldo Moro 500185Rome, Italy.
Mariana GalloLaboratory of Biochemistry and Metabolomics, Department of Medicine and Surgery, University of Parma43125Parma, Italy.ORCID 0000-0002-0022-4499
Thelma A PertinhezLaboratory of Biochemistry and Metabolomics, Department of Medicine and Surgery, University of Parma43125Parma, Italy.
Fabrizio VeticaDepartment of Chemistry, University of Rome, Sapienza, P.le A. Moro 500185Rome, Italy.ORCID 0000-0002-7171-8779
Mauro GiustiniDepartment of Chemistry, University of Rome, Sapienza, P.le A. Moro 500185Rome, Italy.ORCID 0000-0001-9390-0240
Marco D'AbramoDepartment of Chemistry, University of Rome, Sapienza, P.le A. Moro 500185Rome, Italy.ORCID 0000-0001-6020-8581

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The mechanism of the spontaneous deamidation reaction of the Asn residue in solution has been disclosed through a combination of experimental and computational methods. Utilizing the dipeptide Ace-AsnGly-NH2 (AsnGly) as a model system, the free energy barriers of the reaction steps have been modeled in parallel with time-resolved measurements of all species involved, obtained by NMR and IR spectroscopy. The convergent results of the theoretical and experimental data provide a quantitative description of all the kinetic constants for this four-step reaction. Furthermore, this study lends support to the two-step mechanism hypothesis for succinimide formation proposed in the literature and untangles the molecular origin of the reaction's pH dependence. By bridging atomic structure to reaction kinetics, this work paves the way for the next-generation prediction algorithms, which are essential for the advancement of biologics.

Indexed as

AmidesAsparagineDipeptidesHydrogen-Ion ConcentrationKineticsModels, MolecularThermodynamicsAmidesAsparagineDipeptides

Identifiers

PMID42584574
PMCPMC13474922

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.