Evidence map›Paper›PMID 42584559›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026

Applicability of HILIC-SPE pretreatment for the quantitation of N

Hiroya Murakami, Ibuki Sakai, Soo Takasu, Yukihiro Esaka, Yoshinori Inoue, Norio Teshima

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Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Hiroya MurakamiDepartment of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-Cho, Toyota, Japan. hmurakami@aitech.ac.jp.
Ibuki SakaiDepartment of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-Cho, Toyota, Japan.
Soo TakasuGifu Pharmaceutical University, Daigaku-Nishi, Gifu, Japan.
Yukihiro EsakaGifu Pharmaceutical University, Daigaku-Nishi, Gifu, Japan.
Yoshinori InoueDepartment of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-Cho, Toyota, Japan.
Norio TeshimaDepartment of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-Cho, Toyota, Japan.

Funding

JSPS KAKENHI 0K05571JSPS KAKENHI 23H01993JSPS KAKENHI 23K04814
6 · The paper itself

Abstract

DNA adducts analysis is crucial for elucidating the mechanism of mutagenesis and carcinogenesis induced by various chemical compounds. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-MS/MS) is commonly employed to determine DNA adduct. Pretreatment is essential in DNA adduct analysis due to a significant concentration disparity between normal 2'-deoxynucleosides (dNs) and DNA adducts. The level of DNA adducts is typically assessed by the ratio of DNA adducts to normal 2'-deoxyguanosine (dG) when the DNA adducts originate from dG. Consequently, the determination of dG and DNA adducts is necessary for accurate evaluation. Reversed-phase (RP)-solid-phase extraction (SPE) is the first choice for pretreatment of DNA adducts analysis. This pretreatment using RP-SPE effectively enriches DNA adducts while removing a substantial amount of dNs, particularly thymidine (T) and 2'-deoxyadenosine (dA), which may suppress the ionization of DNA adducts in the electrospray ionization (ESI) process. However, two separate measurements are required to determine the concentrations of dG and DNA adducts, as dG is removed during the SPE process. Therefore, an SPE method that simultaneously extracts dG and DNA adducts is desirable. In this study, we investigated the applicability of hydrophilic interaction chromatography (HILIC)-type SPE sorbents developed by our team, for the pretreatment of DNA adduct analysis. N

Indexed as

DeoxyguanosineHydrophobic and Hydrophilic InteractionsSolid Phase ExtractionChromatography, LiquidDNA AdductsLiquid Chromatography-Mass SpectrometrySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryDeoxyguanosineDNA AdductsN2-ethyl-2'-deoxyguanosineDNA adductHydrophilic interaction chromatographySimultaneous analysisSolid-phase extraction

Identifiers

PMID42584559

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.