Evidence map›Paper›PMID 42582832›Full record

ArticleNational science review2026

Heterogeneous viral-host response at single-cell resolution and viral adaptive mutational atlas in birds and mammals upon H5 influenza virus infection.

Jiahao Zhang, Huaiyuan Cai, Tingting Man, Lihong Huang, Letian Li, Yu Chen, Haixing Yang, Shiping Ding, Yiqun Chen, Ruixuan Zhu and 22 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in National science review, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Jiahao ZhangKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Huaiyuan CaiState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Tingting ManState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Lihong HuangKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Letian LiChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China.
Yu ChenState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Haixing YangState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Shiping DingKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Yiqun ChenKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Ruixuan ZhuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Zhengze WangLingang Laboratory, Shanghai Tech University, Shanghai 200031, China.
Qian ZhangCollege of Bioinformatics, Huazhong Agricultural University, Wuhan 430070, China.
Jinjun LiuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Xiaona ChenKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
He ZhangChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China.
Xinyu ZhuangChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China.
Xiaoshu ZhouCollege of Biomedicine and Health, Huazhong Agricultural University, Wuhan 430070, China.
Hui GuoState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Tao ZhangKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Yi LiuKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Junlong XiongKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Jiangtao ZhouKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Weize XuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Xiaofeng WuState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Weixin JiaKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Zhangyong NingKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Ke XiaoShenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518107, China.
Jinxia DaiState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Weifeng ShiRuijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.ORCID https://orcid.org/0000-0002-8717-2942
Wenbao QiKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.ORCID https://orcid.org/0000-0003-0610-7506
Gang CaoState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.ORCID https://orcid.org/0000-0002-8780-1497
Ming LiaoKey Laboratory of Zoonoses, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

H5 highly pathogenic avian influenza viruses (HPAIVs) are prevalent in birds, causing infection in over 60 mammalian species worldwide. However, species-specific viral-host response at the single-cell level and within-host evolution upon H5 HPAIV infection are poorly understood. Here, by integrating spatial transcriptomics and single-cell genomics technologies, we present a comprehensive single-cell transcriptomic landscape of 1499 669 cells in the lungs from birds (chickens, ducks, and pigeons) and mammals (mice and pigs) upon H5 HPAIV infection. H5 HPAIV primarily infected avian endothelial cells and mammalian epithelial cells, respectively. Among the single-cell transcriptomic lung landscape, H5 HPAIV infection induced significant inflammation in fibroblasts and the shedding of alveolar epithelial cells of chickens upon infection, while the expression levels of cytokine- and inflammation-related genes slightly decreased in fibroblasts and endothelial cells of infected ducks and pigeons, respectively. However, mice only exhibited significantly antiviral response in macrophages and neutrophils, while antiviral response was elevated in most subtype cells of pigs upon infection. Notably, we identified 19 viral non-synonymous intrahost single nucleotide variants in the infected birds and mammals and assessed their molecular characterizations

Indexed as

adaptive mutationscross-speciesH5 subtypehighly pathogenic avian influenza viruslung microenvironmentMiP-seqscRNA-seq

Identifiers

PMID42582832
PMCPMC13458601

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