Temporal and Functional Profiling of the Microbiome of High and Low Nitrogen Content Barley Seed in Silo Storage.

Kalonji A Tshisekedi, Tim Van Den Bossche, Lennart Martens, Pieter De Maayer, Angela Botes

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Article in Food science & nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kalonji A TshisekediSchool of Molecular and Cell Biology University of the Witwatersrand Johannesburg South Africa.ORCID https://orcid.org/0000-0002-0518-1643

Tim Van Den BosscheVIB-UGent Center for Medical Biotechnology, VIB Ghent Belgium.ORCID https://orcid.org/0000-0002-5916-2587

Lennart MartensVIB-UGent Center for Medical Biotechnology, VIB Ghent Belgium.ORCID https://orcid.org/0000-0003-4277-658X

Pieter De MaayerSchool of Molecular and Cell Biology University of the Witwatersrand Johannesburg South Africa.ORCID https://orcid.org/0000-0001-8550-642X

Angela BotesSchool of Molecular and Cell Biology University of the Witwatersrand Johannesburg South Africa.ORCID https://orcid.org/0000-0003-1245-2525

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Barley grain quality is influenced by nitrogen content and storage conditions; however, their impact on the composition and function of the grain microbiome is not well understood. This study combined metataxonomic (16S rRNA and ITS) profiling, metagenome sequencing, and metaproteome analyses to characterize the structure and function of the barley grain microbiome. Grains with high (> 1.5%) and low (< 1.5%) nitrogen content from a single barley cultivar (Kadie) were sampled at harvest and after 3, 6, and 9 months of storage. Amplicon sequencing revealed a community dominated by Proteobacteria, Firmicutes, and Ascomycota, while metagenomics confirmed the abundance of genera such as

Identifiers

PMID42582632

DOI10.1002/fsn3.72179

PMCPMC13458027

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