Evidence map›Paper›PMID 42582095›Full record

ArticleInnovation (Cambridge (Mass.))2026

Structured illumination microscopy for high SNR 3D imaging from millimeter-thick tissues to cellular dynamics.

Mengrui Wang, Manming Shu, Jiajing Yan, Chang Liu, Xiangda Fu, Jingxiang Zhang, Yuchen Lin, Hu Zhao, Yuwei Huang, Dingbang Ma and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Innovation (Cambridge (Mass.)), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Mengrui WangMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Manming ShuMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Jiajing YanMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Chang LiuMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Xiangda FuMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Jingxiang ZhangMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Yuchen LinMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Hu ZhaoChinese Institute for Brain Research, Zhongguancun Life Science Park, Beijing 100086, China.
Yuwei HuangSchool of Basic Medical Sciences, Translational Medicine Institute, Xi'an Jiaotong University, Xi'an 710049, China.
Dingbang MaInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China.
Yifan GeInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China.
Huiwen HaoHuaxia Imaging Technology Co., Ltd., Beijing 102600, China.
Tianyu ZhaoMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Yansheng LiangMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Shaowei WangMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.
Ming LeiMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter, School of Physics, Xi'an Jiaotong University, Xi'an 710049, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional (3D) optical imaging of intact tissues with high spatiotemporal resolution is a highly desirable goal, as it offers visualization of biological structures and processes in their physiological context. Optical sectioning structured illumination microscopy (OS-SIM) stands out among various 3D imaging techniques for its comprehensive superiority, as it offers high spatial resolution, rapid imaging speed, and low phototoxicity. However, under the challenging conditions of dominant background and increased fluorescence scattering in thick tissues, which leads to a decrease in modulation contrast, OS-SIM faces the problem of low signal-to-noise ratio (SNR) and limited imaging depth. We have developed a high SNR OS-SIM strategy for 3D imaging of biological samples ranging from millimeter-thick tissues to cellular dynamics. By enhancing the SNR by ∼10 dB with rapid processing, our method enables imaging of tissue-cleared sample with thickness >2 mm and low light dose for live-cell recording, while also doubling the imaging penetration depth. With our OS-SIM system, we achieve high-quality 3D images of various biological samples, including mouse brains,

Indexed as

optical sectioningstructured illumination microscopythree-dimensional imaging

Identifiers

PMID42582095
PMCPMC13457229

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.