Evidence map›Paper›PMID 42581766›Full record

ArticleMicroscopy research and technique2026

A Deep Learning Pipeline for Cell Segmentation and Viability Quantification in 3D Constructs From Fluorescence Microscopy Images.

Federica Valtellina, Francesco Iannacci, Bianca Maria Colosimo, Mattia Sponchioni

Abstract read
In one paragraph

Article in Microscopy research and technique, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Federica ValtellinaDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta," Politecnico di Milano, Milano, Italy.
Francesco IannacciDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta," Politecnico di Milano, Milano, Italy.ORCID https://orcid.org/0009-0002-6110-4260
Bianca Maria ColosimoDepartment of Mechanical Engineering, Politecnico di Milano, Milano, Italy.ORCID https://orcid.org/0000-0001-6844-2030
Mattia SponchioniDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta," Politecnico di Milano, Milano, Italy.ORCID https://orcid.org/0000-0002-8130-6495

Funding

Fondazione Cariplo 2021-1518
6 · The paper itself

Abstract

With the increasing adoption of 3D cell cultures and bioprinting in biomedical research, there is a growing demand for reliable and accurate monitoring methods. While fluorescence microscopy is widely accepted for 2D cell cultures, when introducing the third dimension it often suffers from signal attenuation and out-of-focus interference, making automated image analysis challenging. In this study, a hybrid pipeline that integrates deep learning and traditional image processing techniques is presented with the aim of establishing an automated tool for cell segmentation and viability assessment in 3D from fluorescence microscopy images. A U

Indexed as

Cell Culture Techniques, Three DimensionalDeep LearningImage Processing, Computer-AssistedImaging, Three-DimensionalBioprintingCell SurvivalHumansHuman Umbilical Vein Endothelial CellsMicroscopy, Fluorescence3D bioprintingautomatic countcell viabilityin‐situ monitoringmachine learning

Identifiers

PMID42581766
PMCPMC13635604

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.