Evidence map›Paper›PMID 42581111›Full record

ArticleAnalytical and bioanalytical chemistry2026

A point-of-care testing platform for pathogen nucleic acid analysis utilizing paper-based extraction and portable qPCR.

Jiahao Li, Chuhan Zhang, Zongdong Ou, Yiran Zhao, Zhirun Ji, Yufan Feng, Ting-Hsuan Chen, Zhugen Yang, Minghui Wu, Ruiqi Mao and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jiahao LiSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Chuhan ZhangSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Zongdong OuHainan Province Fisheries Technology Extension Center, Sanya, PR China.
Yiran ZhaoSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Zhirun JiSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Yufan FengSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Ting-Hsuan ChenDepartment of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong, Special Administrative Region, Hong Kong, 999077, China.
Zhugen YangFaculty of Engineering and Applied Sciences, Cranfield University, Cranfield, MK43 0AL, UK.
Minghui WuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Ruiqi MaoSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Chunyang ZhangSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Rui ChuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Xinyi YangSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China.
Tianmi LiuHainan Province Fisheries Technology Extension Center, Sanya, PR China. ltm600@163.com.
Yanke ShanSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China. shanyk26@njau.edu.cn.
Fei LiuSanya Institute of Nanjing Agricultural University, Joint International Research Laboratory of Animal Health and Food Safety of Ministry of Education, Single Molecule Biochemistry & Biomedicine Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing, 210095, PR China. feiliu24@njau.edu.cn.

Funding

College Students' Innovative Entrepreneurial Training Plan Program 202510307076College Students' Innovative Entrepreneurial Training Plan Program S202510307227the Science and Technology Innovation Project of Sanya City 2022KJCX27
6 · The paper itself

Abstract

Rapid and reliable diagnostic methods are crucial for controlling animal disease outbreaks and reducing mortality, especially when effective drugs or vaccines are unavailable. While real-time quantitative polymerase chain reaction (qPCR) remains the gold standard for nucleic acid detection due to its superior sensitivity and specificity, conventional workflows are inherently cumbersome. Traditional extraction and amplification processes typically require 1.5 to 3 h, with further delays incurred by transporting samples to centralized laboratories. To overcome these limitations, this study introduces a point-of-care testing platform for on-site pathogen diagnostics. This system synergistically combines a centrifuge-free, glass fiber (GF) paper-based nucleic acid extraction card, room-temperature-stable lyophilized qPCR reagents, and a portable qPCR instrument. By eliminating the dependency on bulky benchtop equipment and cold-chain logistics, this platform streamlines lysis, extraction, and amplification, delivering sample-to-answer results within 45 min. The optimized system demonstrated excellent analytical sensitivity, achieving limits of detection (LoDs) of 10 copies/μL for the N gene of porcine epidemic diarrhea virus (PEDV) and 50 copies/μL for the PirA gene of Vibrio parahaemolyticus AHPND (VP

Indexed as

Lyophilized reagentsPaper-based nucleic acid extraction cardPoint-of-care testing platformPortable qPCR instrument

Identifiers

PMID42581111

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.