ArticleAnalytical and bioanalytical chemistry2026
Multi-class UHPLC-MS/MS method for the simultaneous determination of 32 endocrine-disrupting chemicals in the urine of pregnant women.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
Endocrine-disrupting chemicals (EDCs) comprise structurally diverse contaminants that are frequently detected in human biological matrices and may interfere with endocrine homeostasis even at low exposure levels. Developing practical multi-residue methods for biomonitoring remains challenging because EDC classes differ markedly in polarity, ionization behavior, and urinary matrix susceptibility. In this study, we developed and validated an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of 32 target EDCs in urine from pregnant women, including 14 bisphenols, 8 per- and polyfluoroalkyl substances (PFAS), 5 organophosphate ester metabolites, and 5 preservatives. Sample preparation involved enzymatic hydrolysis followed by liquid-liquid extraction with ethyl acetate. The method was evaluated using matrix-matched calibration in artificial urine and isotope-labelled internal standards where available. Most analytes showed acceptable linearity, accuracy, precision, matrix effect, extraction efficiency, and stability according to commonly used bioanalytical validation criteria. Limits of quantification ranged from 0.001 to 0.4 ng/mL. The method was further applied to 188 urine samples from pregnant women, demonstrating its applicability for routine multi-class EDC biomonitoring. Although sensitivity for several phenolic analytes and extraction efficiency for selected compounds require further improvement, the proposed method provides a practical platform for high-throughput assessment of mixed EDC exposure in epidemiological studies.
Indexed as
Identifiers
42581106What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.