Evidence map›Paper›PMID 42580348›Full record

ArticleCell stem cell2026

Human iPSC-derived heart valve-like assembloids model valve development and disease pathology.

Yuanhang He, Abbas Jalili, Carter B Jones, Haoting He, Lameck Beni, Daniel Lamont, Junqi Hu, Macy Yost, Lance A Davidson, Si-Yang Zheng and 1 more

Abstract read
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In one paragraph

Article in Cell stem cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuanhang HeDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA; Tsinghua Medicine, Tsinghua University, Beijing 100084, China.
Abbas JaliliDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Carter B JonesDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Haoting HeDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA.
Lameck BeniDepartment of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA; Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Daniel LamontDepartment of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Junqi HuDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA; Tsinghua Medicine, Tsinghua University, Beijing 100084, China.
Macy YostDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA.
Lance A DavidsonDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Si-Yang ZhengDepartment of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA; Department of Electrical Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Guang LiDepartment of Cell Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA; Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15260, USA. Electronic address: guangli@pitt.edu.

Funding

Generation of four-chambered hearts through organoid fusionsDP2HL163745 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LI, GUANG · 2021 to 2024
$2.4M
Cellular and Molecular Mechanisms of Atrial Cardiomyocyte Lineage Commitment R00HL133472 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LI, GUANG · 2019 to 2021
$750k
High-Throughput Computing for Genomics and Bioinformatics ResearchS10OD028483 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI LEE, ADRIAN V · 2021 to 2021
$574k
NHLBI NIH HHS DP2 HL163745NHLBI NIH HHS R00 HL133472NIH HHS S10 OD028483
6 · The paper itself

Abstract

Heart valves maintain unidirectional blood flow, yet most understanding of their development and disease comes from animal models that do not fully capture human valve behavior. We present a human induced pluripotent stem cell (iPSC)-derived valve-like assembloid platform that models key aspects of in vivo valve features at the cellular and molecular levels. We found that mechanical forces, endothelial culture conditions, and fluidic shear stress respectively promote valve induction, maintenance, and extracellular matrix stratification. We further used this system to model human valve defects, including genetic mutations, injury, and hyperglycemia-related abnormalities. This assembloid platform enables the in vitro study of human valve development and disease mechanisms.

Indexed as

Heart Valve DiseasesHeart ValvesInduced Pluripotent Stem CellsModels, BiologicalCell DifferentiationEndothelial CellsExtracellular MatrixHumansStress, Mechanicalcongenital valve deficiencyendothelial cellendothelial-to-mesenchymal transitionhyperglycemiamechanical forceorganoidvalvevalve injury

Identifiers

PMID42580348
PMCPMC13496497

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.