Protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs.

Abdouramane Camara, Baizhi Chen, An Qi Xu, Dinis Pedro Calado

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Abdouramane CamaraImmunity and Cancer Laboratory, The Francis Crick Institute, London, UK; West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, Ghana. Electronic address: abdou.camara@crick.ac.uk.

Baizhi ChenImmunity and Cancer Laboratory, The Francis Crick Institute, London, UK.

An Qi XuImmunity and Cancer Laboratory, The Francis Crick Institute, London, UK.

Dinis Pedro CaladoImmunity and Cancer Laboratory, The Francis Crick Institute, London, UK; Immunity and Cancer Laboratory, The Babraham Institute, Babraham Research Campus, Cambridge, UK; Immunology Programme, The Babraham Institute, Babraham Research Campus, Cambridge, UK. Electronic address: dinis.calado@crick.ac.uk.

Funding

Wellcome Trust CC2078

6 · The paper itself

Abstract

Bone marrow plasma cells maintain durable antibody responses, but their tissue localization and morphology are difficult to quantify in situ. Here, we present a protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs. We describe steps for fixation, optimal cutting temperature (OCT) coumpound embedding, longitudinal opening of mouse femurs, whole-mount immunostaining, optical clearing, and confocal 2D/3D imaging. We detail an Imaris-based workflow for reproducible single-cell segmentation, isotype-resolved counting, and the extraction of morphological features from defined regions of interest.

Indexed as

Cell BiologyImmunologyMicroscopy

Identifiers

PMID42579555

DOI10.1016/j.xpro.2026.104775

PMCPMC13487398

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.