ArticleVeterinary medicine and science2026
Characterization of Phylogroups, Virulence Factors and Antimicrobial Resistance in Canine Pyometra-associated Escherichia coli.
Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of multidrug-resistant extraintestinal pathogenic Escherichia coli (ExPEC) in pets poses a significant threat to animal health and represents a potential source of antimicrobial resistance with zoonotic transmission to humans. A total of 56 pyometra swab samples from dogs were analysed for the presence of E. coli using standard microbiological procedures. Phylogrouping of the E. coli isolates was performed using the quadruplex, and 12 virulence genes were detected by single polymerase chain reaction (PCR) method. Antimicrobial susceptibility testing was conducted against 12 antimicrobial agents in five different classes using the Kirby-Bauer disc diffusion method. E. coli was isolated from all 56 swab samples of pyometra cases using 5% ovine blood agar and MacConkey agar, and identified with Eosin Methylene Blue agar, Methyl Red and Voges-Proskauer tests. fimH was found in all strains. The most common virulence genes were fyuA (96.4%), usp (83.9%), sfa (73.2%), hlyA (69.6%), cnf1 (69.6%), papGIII (64.2%), and papC (58.9%). The least common virulence gene was iucD,while none of the strains carried afa and papG allelic variants (papGI and II). A total of 83.9% of isolates belonged to phylogenetic group B2. All isolates were resistant to amoxicillin/clavulanic acid, ampicillin and lincomycin but sensitive to amikacin. The multidrug resistance (MDR) rate was 58.9% (33/56 isolates). MDR and multiple antibiotic resistance index (MARI) scores were similar between the groups (p > 0.05). The phylogenetic analysis revealed that resistant isolates were found across all detected phylogroups, with strains from different phylogroups sharing the same virulence genes. E. coli from pyometra cases in companion animals is largely associated with resistance and possesses virulence genes enabling efficient colonization and carriage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.