ArticleNucleic acids research2026
Duplication of specialized cis-acting elements SLI and SLIII in the viral RNA 3'UTR enables mosquito-borne orthoflavivirus adaptation to avian hosts.
Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cross-species transmission of mosquito-borne orthoflaviviruses (MBFVs) relies on multi-host switching and adaptation, yet the underlying mechanisms remain incompletely understood. Domain I of the 3'-untranslated region (UTR) is highly structured and shaped by host-driven selection. Mammalian MBFVs usually have two functional stem-loops (SLs), while avian MBFVs possess two extra SLs (SLI and SLIII), whose functions remain unclear. Using Tembusu virus (TMUV) and Japanese encephalitis virus (JEV), we mutated Domain I and characterized recombinant viruses. TMUV mutants with only one SL showed attenuated replication, with efficiency dependent on SL identity. Duplicating SLII boosted TMUV replication in mammalian and avian cells but not in mosquito cells, indicating that repeated SLs contribute to host-specific proliferation. Deleting SLI and/or SLIII impaired TMUV replication in avian cells but not mammalian cells, and dual deletion significantly reduced virulence in ducklings but had limited impact in mice. Replacement of JEV SLI/SLIII with homologous elements derived from a duck-associated strain improved its proliferation in avian cells. Mechanistically, SLI/SLIII regulate TMUV replication in avian cells by interacting with the host factor CELF2, which specifically facilitates viral replication in avian cells. Collectively, SLI and SLIII are important determinants of avian host proliferation in MBFVs, improving understanding of their multi-host ecology and transmission.
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