Evidence map›Paper›PMID 42578369›Full record

ArticleNucleic acids research2026

Ctf18-dependent localization of interstitial telomeric sequence to nuclear pore complexes prevents chromosome fragility.

Mohamad Al Hajj, Manon Pierre, Anissia Ait Saada, Fernando R Rosas Bringas, Marion Dubarry, Stéphane Coulon, Vincent Géli, Sarah A E Lambert, Michael Chang, Dmitri Churikov and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mohamad Al HajjMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.
Manon PierreMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.
Anissia Ait SaadaInstitut Curie, PSL Research University, CNRS, UMR3348, U1368, 91405 Orsay, France.
Fernando R Rosas BringasEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9713 AV Groningen, The Netherlands.
Marion DubarryMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.ORCID 0000-0001-5648-5770
Stéphane CoulonMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.ORCID 0000-0001-8090-914X
Vincent GéliMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.ORCID 0000-0002-4103-7462
Sarah A E LambertInstitut Curie, PSL Research University, CNRS, UMR3348, U1368, 91405 Orsay, France.ORCID 0000-0002-1403-3204
Michael ChangEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9713 AV Groningen, The Netherlands.ORCID 0000-0002-1706-3337
Dmitri ChurikovMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.ORCID 0000-0003-1127-8852
Marie-Noelle SimonMarseille Cancer Research Center (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University, Institut Paoli-Calmettes, 27 bd Leï Roure, 13009 Marseille, France.ORCID 0000-0003-1622-1417

Funding

Agence Nationale de Recherche ANR-19-CE12-0023Agence Nationale de Recherche ANR-23-CE12-0007Consejo Nacional de Ciencia y Tecnología scholarshipDutch Research CouncilLa Ligue Nationale contre le CancerLigue Nationale Contre le Cancer
6 · The paper itself

Abstract

Nuclear pore complexes (NPCs) have emerged as hubs in the spatial regulation of DNA damage repair, including restart of stalled replication forks. Here, we used chromatin endogenous cleavage to show that interstitial telomeric sequence (ITS) and natural telomeres interact with the NPCs during their replication in telomerase-positive cells This localization is triggered by replication fork pausing induced by tight Rap1 binding to ITS and requires the alternative PCNA loader Ctf18, consistent with its interaction with the nucleoporin Nup170 in S phase. Mrc1, acting in the same epistatic group with Ctf18 in sister chromatid cohesion establishment during DNA replication, and the cohesin loader Scc2 contribute to localization to NPCs suggesting cohesin involvement downstream of Ctf18. Pol2 SUMOylation by the SUMO ligase Mms21 also participates in this process in epistasis with Scc2. We propose that SUMOylation and establishment of sister chromatid cohesion behind stalled forks mediate Ctf18-dependent localization to NPCs. Chromosome fragility assays show that the NPC environment protects the forks paused at ITS from homologous recombination-induced breakage, thus favoring their error-free restart.

Indexed as

Chromosome FragilityNuclear PoreSaccharomyces cerevisiae ProteinsTelomereCell Cycle ProteinsChromatidsChromosomal Proteins, Non-HistoneCohesinsDNA ReplicationNuclear Pore Complex ProteinsSaccharomyces cerevisiaeSUMO-1 ProteinSumoylationTelomere-Binding ProteinsCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsCTF18 protein, S cerevisiaeMms21 protein, S cerevisiaeNuclear Pore Complex ProteinsSaccharomyces cerevisiae ProteinsSUMO-1 ProteinTelomere-Binding Proteins

Identifiers

PMID42578369
PMCPMC13458399

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.