Evidence map›Paper›PMID 42577401›Full record

ArticleFrontiers in plant science2026

Raman spectroscopy enables non-destructive quantification of nitrate in C3 and C4 plants.

Savitha Dhandapani, Jayapal Praveen Kumar, Alice Mei Xien Tan, Bhushan Vishal, Valiya Nadakkakath Agisha, Ekta Jain, Shaik Anwar Ahamed Nabeela Nasreen, Ganga Cheerlavancha Sravanthi, Nam Hai Chua, Gajendra Pratap Singh and 2 more

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Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Savitha Dhandapani *Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Jayapal Praveen Kumar *Disruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Alice Mei Xien TanTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Bhushan VishalTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Valiya Nadakkakath AgishaTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Ekta JainDisruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Shaik Anwar Ahamed Nabeela NasreenTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Ganga Cheerlavancha SravanthiDisruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Nam Hai ChuaTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
Gajendra Pratap SinghDisruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Rajeev J RamDisruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Bong Soo ParkTemasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate assessment of plant nitrate status is critical for growth and productivity, yet early and non-destructive quantification remains challenging. Although Raman spectroscopy has been used to detect nitrate deficiency in plants, quantitative estimation of nitrate concentration from Raman spectra has not been demonstrated. Here, we evaluated whether Raman spectroscopy can be used to quantitatively predict leaf nitrate concentrations during early nitrogen stress. Two-week-old Pak Choi (C3) and Amaranthus (C4) plants were subjected to nitrate deprivation for 1-3 days, and Raman spectra were collected and correlated with nitrate concentrations determined by biochemical assays. A strong linear relationship was observed between nitrate concentration and the intensity ratio of the nitrate-associated Raman peak at 1046 cm

Indexed as

C3 plantsC4 plantsnitrate quantificationnitrogen deficiencynon-destructive sensingRaman spectroscopyspectral biomarkers

Identifiers

PMID42577401
PMCPMC13454117

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