Evidence map›Paper›PMID 42576045›Full record

ArticleAnalytical and bioanalytical chemistry2026

A CbAgo-enabled fluorescence assay for sensitive detection of terminal deoxynucleotidyl transferase.

Zhenyu Hu, Xiangyan Dong, Jiwei Zhang, Xueting Li, Hui Chen, Xiaopeng Wang, Guanghong Xu, Shun Zhu, Zerong Li, Wenli Xia and 1 more

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zhenyu HuInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Xiangyan DongInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Jiwei ZhangInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Xueting LiInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Hui ChenSchool of Chemistry, South China Normal University, Guangzhou, 510006, China.
Xiaopeng WangInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Guanghong XuInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Shun ZhuInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Zerong LiInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Wenli XiaInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Feng LiuInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China. liu.feng@sz.tsinghua.edu.cn.

Funding

Development and Reform Commission of Shenzhen Municipality No. 2019156
6 · The paper itself

Abstract

Terminal deoxynucleotidyl transferase (TdT) has been recognized as an important biomarker for leukemia diagnosis and prognosis, as its abnormal overexpression is closely associated with malignant lymphoid proliferation and genomic instability. Accurate and sensitive detection of TdT activity is therefore of great significance for understanding polymerase-associated biological processes and supporting analytical and therapeutic research. However, conventional TdT detection methods often involve complex multi-step operations, long incubation times, or amplification reactions, which limit their efficiency and practicality. In this work, a one-pot fluorescence assay integrating TdT-mediated elongation with the programmable endonuclease Clostridium butyricum Argonaute (CbAgo) was developed for rapid, sensitive, and specific TdT activity analysis. In this coupled enzymatic cascade, TdT extends the 5'-phosphorylated A10 primer to generate poly(A) sequences, which hybridize with a fluorophore-quencher reporter to trigger CbAgo-catalyzed cleavage and produce a strong fluorescence signal. Benefiting from the DNA-guided, PAM-independent, and highly stable nature of CbAgo, the proposed system enables homogeneous detection with improved robustness compared with CRISPR-based methods. The assay achieves a detection limit of 6.6 × 10⁻

Indexed as

CbAgoFluorescence detectionOne-pot assayTerminal deoxynucleotidyl transferase

Identifiers

PMID42576045

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.