Evidence map›Paper›PMID 42575438›Full record

ArticleThe Journal of biological chemistry2026

Dynamic SOX2 gene expression revealed by phase-separated single RNA imaging in live porcine cells.

Chaoqian Jiang, Zhongyu Yuan, Chuanpei Li, Tianze Li, Jiayan Wu, Dongyan Yang, Xueqing Liu, Zhuoran Yu, Yuchang Yao, Zhonghua Liu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Chaoqian JiangKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Zhongyu YuanKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Chuanpei LiKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Tianze LiKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Jiayan WuKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Dongyan YangKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Xueqing LiuKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Zhuoran YuKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China.
Yuchang YaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Zhonghua LiuKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China. Electronic address: liuzhonghua@neau.edu.cn.
Jun SongKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China. Electronic address: songjun@neau.edu.cn.
Yanshuang MuKey Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin, China; College of Life Science, Northeast Agricultural University, Harbin, China. Electronic address: muyanshuang@neau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA plays a central role in the formation of diverse traits in pigs. Visualizing RNA dynamics at the single-molecule level in living cells is therefore essential for understanding gene-expression regulation in this species. We report an optimized MS2-MCP RNA-labeling system that incorporates a foldon trimerization domain fused to a fluorescent protein and MCP, thereby enhancing fluorescence signals through liquid-liquid phase separation. Using this Foldon-MS2/MCP system, we successfully imaged endogenous GFP mRNA foci in both the nucleus and cytoplasm of living pig cells. Compared with the conventional MS2-MCP system, the Foldon-MS2/MCP system showed markedly increased fluorescence intensity and an improved fluorescence signal-to-noise ratio. We further applied this approach to the endogenous porcine SOX2 gene, achieving high-resolution tracking of SOX2 mRNA foci in live pig cells. Moreover, the system enabled real-time visualization of SOX2 mRNA elongation and translation dynamics. The Foldon-MS2/MCP system provides a powerful platform for investigating RNA localization, gene-expression activation, and endogenous mRNA dynamics in living pig cells.

Indexed as

foldon-MS2/MCP systemMS2/MCP systempigRNA image

Identifiers

PMID42575438
PMCPMC13571416

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.