ArticleBioresources and bioprocessing2026
Improvement of biosurfactant production by Rhodococcus ruber IEGM 231 grown on waste cooking oil, their emulsifying activity and antioxidant potential.
Article in Bioresources and bioprocessing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In recent years, the production of microbial biosurfactants, increasingly used in various industries and agriculture, from low-cost renewable substrates is intensively studied. Large volumes of food waste, in particular waste cooking oil, are promising substrates for the production of biosurfactants on an industrial scale. However, studies on the ability of Rhodococcus bacteria, well-known biosurfactant producers, to grow on this substrate and the functional characteristics of synthesized biosurfactants are still scarce. In this study, the possibility of improving biosurfactant production by Rhodococcus ruber IEGM 231 grown on waste cooking oil was investigated using multifactor analysis and response surface methodology (RSM). Using standardized parameters for the concentrations of carbon and nitrogen sources in the fermentation medium, a Pareto diagram was constructed, which shows a direct dependence of the crude biosurfactant yield on the oil concentration, while the concentrations of sugar and inorganic nitrogen salts were found insignificant. Using RSM, the optimal ratio of the medium parameters was determined for the maximum yield of crude biosurfactants: oil (+ 1), sugar (0), NH
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