ArticleMethods in molecular biology (Clifton, N.J.)2026
Dynamic Control of Cell Signaling with Optogenetic Modulation of Protein Abundance in Living Mammalian Cells.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Precise modulation of protein levels is essential for investigating the dynamics of signaling pathways. Degradation-based approaches allow rapid reduction of protein abundance, and their combination with optogenetic regulation enables controlled and reversible manipulation. In this chapter, we describe an optogenetic strategy that stabilizes a protein of interest by inhibiting its degradation. As a proof of concept, we demonstrate light-dependent stabilization of MKP3 and constitutively active MEK and examine the resulting changes in ERK signaling. We also provide a step-by-step protocol for measuring protein stabilization kinetics. This method integrates a potent degradation tag with optogenetic control to achieve tunable and reversible regulation of protein levels in living cells.
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