Design and Semisynthesis of Ubiquitin Extension Probes.

Chuntong Li, Luyu Shi, Yingyue Zhang, Jinghong Li

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chuntong Li *School of Pharmaceutical Sciences, Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, China Pingyuan Laboratory, State Key Laboratory of Antiviral Drugs, Zhengzhou University, Zhengzhou, Henan, China.

Luyu Shi *Center for BioAnalytical Chemistry, Hefei National Laboratory of Physical Science at Microscale, University of Science and Technology of China, Hefei, China.

Yingyue ZhangCenter for BioAnalytical Chemistry, Hefei National Laboratory of Physical Science at Microscale, University of Science and Technology of China, Hefei, China.

Jinghong LiBeijing Life Science Academy, Beijing, China. jhli@mail.tsinghua.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Activity-based ubiquitin probes serve as crucial tools for capturing and elucidating the molecular mechanisms of E3 ligase-catalyzed substrate ubiquitination. We recently reported a chemoenzymatic strategy for generating ubiquitin chain extension probes, which offers advantages such as mild reaction conditions, simple operation, and high specificity, thereby providing new opportunities for E3 enzyme research. This article details the experimental protocol for obtaining a K48-linked di-ubiquitin extension probe based on a substrate-Type II degron using the chemoenzymatic approach.

Indexed as

Molecular ProbesUbiquitinUbiquitin-Protein LigasesDegronsHumansSubstrate SpecificityUbiquitinationMolecular ProbesUbiquitinUbiquitin-Protein LigasesChemoenzymatic strategyType II degronUbiquitin chain modificationUbiquitin E3 ligaseUbiquitin extension probes

Identifiers

PMID42573691

DOI10.1038/s41467-024-45635-y

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.