Evidence map›Paper›PMID 42573072›Full record

ArticleNucleic acids research2026

Nucleostemin promotes RAD51 filament assembly on double-stranded DNA to protect stalled replication forks.

Chih-Chun Chang, Siang-Sheng Tsai, Chang-Le Chou, Yi-Hsuan Chang, Chia-Tai Hung, Robert Y L Tsai, Hung-Wen Li, Hungjiun Liaw, Peter Chi

Abstract read
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In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chih-Chun ChangInstitute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan.
Siang-Sheng TsaiDepartment of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan.
Chang-Le ChouDepartment of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Yi-Hsuan ChangInstitute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan.
Chia-Tai HungDepartment of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan.
Robert Y L TsaiInstitute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, TX 77030, United States.ORCID 0000-0003-2006-4610
Hung-Wen LiDepartment of Chemistry, National Taiwan University, Taipei 106, Taiwan.ORCID 0000-0002-5614-3727
Hungjiun LiawDepartment of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan.ORCID 0000-0002-3481-709X
Peter ChiInstitute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan.ORCID 0000-0001-9229-8729

Funding

Academia SinicaCancer Prevention and Research Institute of Texas CPRIT RP200081National Science and Technology Council 114-2123-M-002-008National Science and Technology Council NSTC 112-2326-B-002-009National Science and Technology Council NSTC 112-2326-B-006-002National Science and Technology Council NSTC 113-2311-B-006-004National Science and Technology Council NSTC 114-2326-B-002-006National Taiwan University
6 · The paper itself

Abstract

Replication stress threatens genome integrity by stalling replication forks, which may lead to double-stranded DNA (dsDNA) breaks. Stalled replication forks can be rescued through the fork reversal mechanism, yet they remain vulnerable to nucleolytic attack. Here, we identify nucleostemin (NS/GNL3) as a critical fork-stabilizing factor. We show that NS rapidly accumulates at hydroxyurea-stalled forks and is indispensable for protecting reversed forks, with NS depletion abolishing RAD51 foci formation and unleashing MRE11-mediated degradation. Through biochemical reconstitution and single-molecule fluorescence resonance energy transfer (FRET), we demonstrate that NS binds DNA directly, engages RAD51, and promotes nucleoprotein filament assembly on the dsDNA segment adjacent to the single-stranded DNA/dsDNA junction. This NS-RAD51 complex synergistically shields nascent strands from MRE11-mediated nucleolytic attack. Our work uncovers a new mechanism of fork protection and positions NS as a key guardian of genome integrity under replication stress.

Indexed as

DNADNA ReplicationGTP-Binding ProteinsNuclear ProteinsRad51 RecombinaseDNA-Binding ProteinsDNA, Single-StrandedHumansHydroxyureaMRE11 Homologue ProteinDNADNA-Binding ProteinsDNA, Single-StrandedGNL3 protein, humanGTP-Binding ProteinsHydroxyureaMRE11 Homologue ProteinMRE11 protein, humanNuclear ProteinsRAD51 protein, humanRad51 Recombinase

Identifiers

PMID42573072
PMCPMC13454837

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.