Evidence map›Paper›PMID 42572066›Full record

ArticleScientific reports2026

A sensitive and green spectrofluorimetric method for determination of guanfacine hydrochloride based on NBD-Cl derivatization.

Aamal A Al-Mutairi, Saleh I Alaqel, Farooq M Almutairi, Maram H Abduljabbar, Manal E Alosaimi, Arwa S Alqahtani, Sherif Ramzy, Atiah H Almalki

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Aamal A Al-MutairiDepartment of Chemistry, Faculty of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box, 90950, Riyadh, 11623, Saudi Arabia.
Saleh I AlaqelDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Northern Border University, Rafha, 91911, Saudi Arabia.
Farooq M AlmutairiDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, University of Hafr AlBatin, Hafr AlBatin, 39524, Saudi Arabia.
Maram H AbduljabbarDepartment of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif, 21944, Saudi Arabia.
Manal E AlosaimiDepartment of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Arwa S AlqahtaniDepartment of Chemistry, Faculty of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box, 90950, Riyadh, 11623, Saudi Arabia.
Sherif RamzyPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al- Azhar University, Nasr City, 11751, Cairo, Egypt. ssherif14@azhar.edu.eg.
Atiah H AlmalkiDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif, 21944, Saudi Arabia.

Funding

Princess Nourah Bint Abdulrahman University PNURSP2026R466
6 · The paper itself

Abstract

A sensitive and environmentally friendly spectrofluorimetric method was developed for the determination of guanfacine in pharmaceutical formulations. The method is based on derivatization of guanfacine with 4-chloro-7-nitrobenzofurazan in alkaline medium to yield a highly fluorescent product measured at 535 nm after excitation at 466 nm. Experimental conditions affecting the reaction were systematically optimized to achieve maximum fluorescence intensity. The method exhibited excellent linearity over the concentration range of 50-500 ng/mL with a correlation coefficient of 0.9994. The limits of detection and quantitation were found to be 13.80 and 41.82 ng/mL, respectively, indicating high sensitivity. The method was validated in accordance with ICH guidelines and demonstrated satisfactory accuracy, precision, robustness, and selectivity. It was successfully applied to the analysis of guanfacine in commercial tablet formulations without interference from excipients. Greenness assessment using Analytical Eco-scale and AGREE tools confirmed the environmentally benign nature of the method, achieving a high Eco-scale score of 89 and an AGREE score of 0.68. Compared with a reported method, the proposed approach offers improved sensitivity and superior environmental benignity, making it suitable for routine quality control analysis.

Indexed as

4-Chloro-7-nitrobenzofurazanGuanfacineLimit of DetectionReproducibility of ResultsSpectrometry, FluorescenceTablets4-Chloro-7-nitrobenzofurazan7-nitrobenzo-2-oxa-1,3-diazole chlorideGuanfacineTabletsGreen analytical chemistry.GuanfacineNBD-Cl derivatizationSpectrofluorimetry

Identifiers

PMID42572066
PMCPMC13454439

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.