ArticleSTAR protocols2026
Protocol for live-imaging of blood flow and vascular permeability in zebrafish larvae.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors.
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Abstract
Cerebral vascular dynamics are critical to maintain brain homeostasis, and the fenestrated capillaries of the hypophysis (also known as the pituitary) exhibit unique structural and functional features that support brain-to-periphery neuroendocrine communication. Here, we present a protocol for live imaging of the dynamics of blood flow velocity, capillary diameter, and permeability of the hypophyseal vasculature in awake zebrafish larvae. We describe steps for two-photon microscopy-based data acquisition and the subsequent procedure for quantitative analysis of these vascular parameters. For complete details on the use and execution of this protocol, please refer to Rajamannar et al.
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