ArticleMacromolecular bioscience2026
Optimization of Dual-Bacteriophage Magnetic Nanoprobe for the Detection of Staphylococcus aureus and Pseudomonas aeruginosa.
Article in Macromolecular bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Due to the high host specificity of bacteriophages, a single-phage superparamagnetic nanoprobe can only detect one bacterial species at a time. In this study, we developed a series of dual-phage magnetic nanoprobes and performed a series of evaluations to identify the optimal formulation. Phages specific to Staphylococcus aureus and Pseudomonas aeruginosa were chemically conjugated to superparamagnetic iron oxide nanoparticles. The lytic activity of each phage was assessed individually and in combination. Six single-phage nanoprobes and nine dual-phage nanoprobes with different phage loading levels were prepared. The lowest detectable bacterial concentration and the number of bacteria captured by each probe were subsequently determined. We developed a dual-phage superparamagnetic nanoprobe with high sensitivity and a detection limit of 10
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