ArticleFrontiers in microbiology2026
Detection and colocalization of STEC genetic markers in wheat flour using whole cell digital droplet PCR.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Shiga toxin-producing Methods: We optimized the ddPCR protocol for the detection and colocalization of critical STEC genetic markers, namely Results: Both qPCR and ddPCR analytical workflows were shown to have a similar LOD50 around 0.9 CFU/g in wheat flour under the tested conditions. Our colocalization analysis was successful in wheat flour inoculated with single strains or a pool of two strains with different genetic make-ups at different inoculation levels. Furthermore, we were able to show detection and genetic association patterns by ddPCR across naturally contaminated retail flour samples. Discussion: This flexible whole cell ddPCR approach addresses significant limitations of existing STEC detection methods and provides a useful data set to support the development of a more sophisticated molecular analytical method which can be applied to wheat flour to detect and characterize STEC strains.
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