Evidence map›Paper›PMID 42566308›Full record

ArticleSTAR protocols2026

Protocol to generate human T cell progenitors that repopulate the thymus.

Elena S Philonenko, Baoyun Zhang, Eugene Albert, Zahir Shah, Peng Li, Pavel Volchkov, Igor M Samokhvalov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Elena S PhilonenkoCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. Electronic address: elenaphilonenko@tym-job.com.
Baoyun ZhangCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Eugene AlbertFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, 125315 Moscow, Russia; Moscow Center for Advanced Studies, 123592 Moscow, Russia.
Zahir ShahDepartment of Hematology & Hematopoietic Cell Transplantation, City of Hope National Medical Center, Los Angeles, CA 91010, USA; Hematologic Malignancies Research Institute, City of Hope National Medical Center, Los Angeles, CA 91010, USA.
Peng LiCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Pavel VolchkovFederal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, 125315 Moscow, Russia; Faculty of Fundamental Medicine, Lomonosov Moscow State University, 119991 Moscow, Russia; Moscow Center for Advanced Studies, 123592 Moscow, Russia.
Igor M SamokhvalovCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, 125315 Moscow, Russia. Electronic address: igormikhail@aol.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) provide a powerful platform for generating T cell progenitors and investigating hematopoietic stem cell (HSC)-independent T cell development. Here, we present a protocol for the directed differentiation of hPSCs to generate early progenitors of human T cells that repopulate the thymus long term and gradually develop into mature T cells in vivo. The protocol includes primary hematopoietic differentiation of hPSCs in defined culture conditions, a secondary differentiation step to prime T lymphopoiesis, and mouse transplantation procedures. For complete details on the use and execution of this protocol, please refer to Philonenko et al.

Indexed as

Cell BiologyCell cultureCell DifferentiationDevelopmental biologyFlow CytometryImmunologyStem Cells

Identifiers

PMID42566308
PMCPMC13476364

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.