Protocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slices.

Patrick Cottilli, Eugene V Mosharov, Michael J Devine, David L Sulzer

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Patrick CottilliMitochondrial Neurobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. Electronic address: patrick.cottilli@crick.ac.uk.

Eugene V MosharovColumbia University, New York, New York, NY 10027, USA; New York State Psychiatric Institute, New York, New York, NY 10032, USA.

Michael J DevineMitochondrial Neurobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK; Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, University College London, London WC1N 3BG, UK.

David L SulzerColumbia University, New York, New York, NY 10027, USA; New York State Psychiatric Institute, New York, New York, NY 10032, USA. Electronic address: ds43@cumc.columbia.edu.

Funding

Wellcome Trust CC2206

6 · The paper itself

Abstract

Striatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluorescent false neurotransmitter 200 (FFN200), a vesicular monoamine transporter 2 (VMAT2) substrate accumulated by and released from DA synaptic vesicles, providing spatial and temporal kinetics of exocytosis. We describe steps for preparing mouse acute brain slices, loading slices with the FFN, acquiring images with 2-photon microscopy, and analyzing the data. For complete details on the use and execution of this protocol, please refer to Hwu et al.

Indexed as

MicroscopyMolecular/Chemical ProbesNeuroscience

Identifiers

PMID42566307

DOI10.1016/j.xpro.2026.104773

PMCPMC13476366

What OpenQuestion holds

Textmetadata

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.