Evidence map›Paper›PMID 42565895›Full record

ArticleActa parasitologica2026

Genetic Profiling of Leishmania Species Causing Human Visceral Leishmaniasis in Algeria.

Houria Zait, Tahar Kernif, Fayez Ahmed Khardine, Asmaa Sekkai, Amina Boutellis, Aïssam Hachid, Boussaad Hamrioui, Sihem Benaissa, Naouel Eddaikra

Abstract read
PubMed Publisher
In one paragraph

Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Houria ZaitFaculty of Pharmacy, University of Health sciences, 36 Street Château Neuf, Benaknoun, 16002, Algiers, Algeria. zaithouria@gmail.com.
Tahar KernifLaboratory of Ecology and Epidemiology and Genetics of Parasitic Populations, Pasteur Institute of Algeria, Street of petit Staoueli, Dely-Brahim, 16048, Algiers, Algeria.
Fayez Ahmed KhardineLaboratoire des Arbovirus et Virus Emergents, Pasteur Institute of Algeria, SidiFredj, 16096, Algiers, Algeria.
Asmaa SekkaiLaboratory of Biodiversity and Environment: Interactions-Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16042, Algiers, Algeria.
Amina BoutellisLaboratory of Biodiversity and Environment: Interactions-Genomes, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, 16042, Algiers, Algeria.
Aïssam HachidFaculty of Pharmacy, University of Health sciences, 36 Street Château Neuf, Benaknoun, 16002, Algiers, Algeria.
Boussaad HamriouiParasitology and mycology Department, Mustapha tertiary care hospital, Place du 1er Mai, 16000, Algiers, Algeria.
Sihem BenaissaFaculty of Pharmacy, University of Health sciences, 36 Street Château Neuf, Benaknoun, 16002, Algiers, Algeria.
Naouel EddaikraLaboratory of Ecology and Epidemiology and Genetics of Parasitic Populations, Pasteur Institute of Algeria, Street of petit Staoueli, Dely-Brahim, 16048, Algiers, Algeria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman visceral leishmaniasis (HVL) in Algeria is primarily caused by Leishmania infantum. This study aimed to genetically identify the Leishmania species responsible for HVL in Algerian patients.

methodsThis descriptive molecular case series included 13 patients diagnosed between 2010 and 2022. DNA from blood, bone marrow, and skin lesion smears was analyzed using ITS1-PCR, RFLP analysis and Sanger sequencing. The resulting sequences were compared with GenBank references, and phylogenetic analyses were performed.

resultsL. infantum was identified in 11 of 13 cases (84.6%). Most visceral leishmaniasis cases occurred in patients younger than 16 years, whereas adult cases were primarily immunocompromised patients, particularly those living with HIV/AIDS. Phylogenetic analyses revealed a high degree of similarity between Algerian L. infantum isolates and Mediterranean strains. In addition to L. infantum, L. tropica DNA and, surprisingly, L. major DNA was also identified. L. major DNA was detected in one immunocompetent child, while L. tropica DNA was detected in a skin lesion of an immunocompromised adult who presented with a concomitant relapse of HVL. These atypical molecular findings require cautious interpretation and further investigation.

conclusionsL. infantum remains the principal causative agent of HVL in Algeria. The atypical molecular findings should be interpreted cautiously and highlight the need for continued molecular surveillance, larger studies incorporating additional genetic markers, and investigations of vector dynamics and animal reservoirs.

Indexed as

Genetic ProfileLeishmaniaLeishmaniasis, VisceralAdolescentAdultAgedAlgeriaChildChild, PreschoolDNA, ProtozoanFemaleHumansInfantLeishmania infantumMaleMiddle AgedDNA, ProtozoanAlgeriaHuman visceral leishmaniasisLeishmania infantumMolecular identification

Identifiers

PMID42565895

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.