Evidence map›Paper›PMID 42564716›Full record

ArticleMethodsX2026

Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay.

Codey E Dueck, Nataliya Santiago, Nikho A Hizon, Ravinder Lidder, Dave Spreitzer, Umar P Mohammed, MacKenzie Dyck, Chand S Mangat, Edgard M Mejia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Codey E DueckWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Nataliya SantiagoWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Nikho A HizonWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Ravinder LidderWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Dave SpreitzerWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Umar P MohammedWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
MacKenzie DyckWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Chand S MangatWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.
Edgard M MejiaWastewater Surveillance Unit, Scientific Core Services Division, National Microbiology Laboratory, Public Health Agency of Canada, 745 Logan Avenue, Winnipeg, Manitoba, R3E 3L5, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bovine coronavirus (BCoV) is commonly used to evaluate the efficiency of nucleic acid extraction protocols used in wastewater-based surveillance (WBS). Here we outline a method for measuring the efficiency of a solids-based extraction protocol using RT-qPCR detections of BCoV spiked into wastewater samples. Validation steps included: determination of a suitable quantification method for BCoV starting material, measures of endogenous BCoV in wastewater, assessment of the effects of BCoV incubation times on wastewater extraction efficiencies, and implementation of a BCoV and Pepper Mild Mottle Virus (PMMoV) RT-qPCR duplex assay. Results show that an accurate baseline measure of BCoV stock concentrations is best achieved using heat lysis extraction compared to an automated method. We also show that endogenous BCoV can be present in wastewater, albeit at low viral loads. Additionally, a BCoV incubation time of 16 h in wastewater resulted in the highest percent recovery. Lastly, the use of the BCoV and PMMoV RT-qPCR duplex assay demonstrates comparable performance to their singleplex counterparts. • A method for measuring the extraction efficiency of a solids-based wastewater extraction process using BCoV. • Insights into the in situ prevalence of BCoV in wastewater samples. • Assessment of a multiplex assay for simultaneous detection of BCoV and PMMoV.

Indexed as

Endogenous controlNucleic acid extractionQuality controlSurrogate recovery controlWastewater-based surveillance

Identifiers

PMID42564716
PMCPMC13446042

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.