Evidence map›Paper›PMID 42563555›Full record

ArticlePlant biotechnology journal2026

Natural and CRISPR/Cas9 Editing Variations of Dual-uORFs Synergistically Increase the Nutritional Value of Silage Maize Feed.

Jing Zhou, Xi Wang, Yajing Zhang, Xinhao Luo, Shuyao Quan, Jie Ji, Lixue Sun, Xinyang Chen, Jin Chen, Jianbo Cheng and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jing ZhouThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.ORCID https://orcid.org/0009-0001-7430-9831
Xi WangThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Yajing ZhangThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Xinhao LuoThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.ORCID https://orcid.org/0000-0001-6908-7456
Shuyao QuanThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Jie JiThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Lixue SunThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Xinyang ChenThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Jin ChenThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.ORCID https://orcid.org/0009-0003-6741-6705
Jianbo ChengCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Jun FanThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Changsheng LiThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Beijiu ChengThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
Xiaoyu LiThe National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.

Funding

Anhui Provincial Key Research and Development Program 2023n06020023Bio-breeding Laboratory of Anhui Province 2025SWYZ0200
6 · The paper itself

Abstract

The precise enhancement of nutritional quality in silage maize is a core strategy for increasing livestock production efficiency. Through evolutionary analysis of multiple plant species, we identified two functionally synergistic upstream open reading frames (uORF1 and uORF2) within the 5' untranslated region of the gene encoding GDP-L-galactose phosphorylase (GGP). By leveraging a natural translation-enhancing haplotype of uORF1 (Hap2) and performing CRISPR/Cas9-mediated targeted mutagenesis of the highly conserved uORF2, we successfully engineered an elite dual-uORFs variant. This variant significantly increased vitamin C (Vc) content and concurrently improved key silage quality traits, including crude protein and phosphorus levels, without inducing growth penalties. Transcriptomic profiling further elucidated the molecular mechanisms by which the dual-uORFs variation coordinately regulates Vc biosynthesis and the improvement of silage quality. Our findings deepen the understanding of the conventional paradigm of single-uORF regulation and provide a novel strategy and superior germplasm resources for the precision breeding of high-Vc, high-quality silage maize.

Indexed as

dual‐uORFsGGPhaplotypenutritional qualitysilage maizeVc

Identifiers

PMID42563555
PMCPMC13448561

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.