Evidence map›Paper›PMID 42563056›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Precise and Stable Labeling of Quantum Dots with Engineered Antibodies.

Victor R Mann, Cassio C S Pedroso, Bruce E Cohen

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Victor R MannThe Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Cassio C S PedrosoThe Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Bruce E CohenThe Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. becohen@lbl.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantum dots (QDs) are ideal probes for quantitative bioimaging but require controlled surface functionalization to be useful for imaging specific receptors or other cellular targets. Antibodies (Ab) offer high affinities and specificities against a range of targets, but methods for synthesizing QD-Ab conjugates may offer little control over stoichiometry, orient Ab incorrectly, or be reversible or denaturing. Here, we describe a protocol using SpyCatcher proteins, which spontaneously form covalent bonds with paired SpyTag peptides, to stably link Ab to QD surfaces with control over stoichiometry and orientation. Protocols for immunolabeling of CdSe/CdS QDs with miniaturized Ab, their characterization, and their use in live cell imaging are presented.

Indexed as

AntibodiesProtein EngineeringQuantum DotsSingle-Chain AntibodiesStaining and LabelingAnimalsCadmium CompoundsHumansPeptidesAntibodiesCadmium CompoundsPeptidesSingle-Chain AntibodiesSpyTag peptideAqueous passivationBioconjugationImmunotargetingMicroscopyProtein engineeringReceptorScFv antibody

Identifiers

PMID42563056

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.