Evidence map›Paper›PMID 42562982›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Enzymatic Bioautographic Methods.

I Ayelen Ramallo, Mario O Salazar, Ricardo L E Furlan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

I Ayelen RamalloFacultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina.
Mario O SalazarFacultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina.
Ricardo L E FurlanFacultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina. furlan@rosario-conicet.gov.ar.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate, making it particularly useful for identifying inhibitors in complex mixtures. This technique allows the direct analysis of the inhibitory properties of compounds separated by thin-layer chromatography, generating an inhibition profile. Here, we describe the conditions to detect inhibitors of the xanthine oxidase and β-glucosidase immobilized on agar gel.

Indexed as

beta-GlucosidaseEnzyme InhibitorsXanthine OxidaseChromatography, Thin LayerEnzymes, Immobilizedbeta-GlucosidaseEnzyme InhibitorsEnzymes, ImmobilizedXanthine OxidaseBioautography-based screeningBioguided isolationControl assayImmobilization on gel matrixXanthine oxidaseβ-glucosidase

Identifiers

PMID42562982

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.