Evidence map›Paper›PMID 42562979›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors.

Abraham Ochoa, Frida Trejo, Luis F Olguín

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Abraham OchoaLaboratorio de Biofísicoquímica. Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México.
Frida TrejoLaboratorio de Biofísicoquímica. Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México.
Luis F OlguínLaboratorio de Biofísicoquímica. Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, México. olguin.lf@comunidad.unam.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sub-nanoliter droplets produced in microfluidic devices have gained enormous importance for performing all kinds of biochemical assays. One of the main reasons is that the amounts of reagents employed can be reduced by approximately five orders of magnitude compared with conventional microplate assays. This chapter describes how to design, fabricate, and operate a microfluidic device to perform enzyme kinetics and enzyme inhibition assays in droplets. This procedure can be used effectively to screen a small library of compounds. Then, we describe how to use this droplet microfluidic setup to screen for potential inhibitor compounds eluted from a coupled high-performance liquid chromatography (HPLC) system that separates crude natural extracts.

Indexed as

Enzyme AssaysEnzyme InhibitorsMicrofluidic Analytical TechniquesMicrofluidicsChromatography, High Pressure LiquidHigh-Throughput Screening AssaysKineticsEnzyme InhibitorsAssaysDropletDroplet-Based assaysDrug discoveryEnzymatic assaysEnzyme inhibitionEnzyme kineticsFluorescenceHigh-throughput screeningLiquid chromatographyMicrodropletsMicrofluidic deviceMicrofluidicsNatural products

Identifiers

PMID42562979

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.