Evaluation of Endolysin Activity Using Nephelometric Assays with Whole Bacterial Cells and Isolated Peptidoglycan.

Panagiota D Pantiora, Nikolaos D Georgakis, Georgios E Premetis, Christina Varotsou, Eleni Pavlou, Alexandra Sklavou, Irene Axarli, Nikolaos E Labrou

Abstract read

PubMed Publisher

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Panagiota D PantioraLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Nikolaos D GeorgakisLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Georgios E PremetisLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Christina VarotsouLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Eleni PavlouLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Alexandra SklavouLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Irene AxarliLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece.

Nikolaos E LabrouLaboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855, Athens, Greece. Lambrou@aua.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents a set of protocols for evaluating endolysin activity through nephelometric turbidity assays. Nephelometric assays measure the decrease in optical density over time using either heat-inactivated bacterial cells or purified peptidoglycan (PG) as substrates, offering a rapid and quantitative approach to assess lytic enzyme function. Complementary methods for PG extraction, purification, and dye-labeling are also included. Together, these protocols support consistent and reproducible assessment of endolysin activity across diverse experimental contexts.

Indexed as

BacteriaEndopeptidasesEnzyme AssaysNephelometry and TurbidimetryPeptidoglycanendolysinEndopeptidasesPeptidoglycanBacterial cell lysisEndolysin assayEnzyme kineticsGlycosidic bond hydrolysisGram-positive bacteriaNephelometric turbidityPeptidoglycan isolationRemazol brilliant blue

Identifiers

PMID42562977

DOI10.1007/978-1-0716-3778-4_18

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.