ArticleMethods in molecular biology (Clifton, N.J.)2026
Binding Interaction Analysis of Lysozyme and Brilliant Blue Using Surface Plasmon Resonance.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study presents a detailed in vitro experimental protocol for analyzing the molecular interaction between lysozyme and the synthetic dye Brilliant Blue FCF (BB). Lysozyme, a natural antimicrobial enzyme found in various secretions, plays a vital role in the innate immune system and oral health. BB, commonly used in food and cosmetic products, is known to interact with biological macromolecules. The binding interaction between these two molecules was investigated using a surface plasmon resonance (SPR) system employing Kretschmann's configuration. The gold SPR sensor surface was functionalized with 11-mercaptoundecanoic acid (MUA). The lysozyme from chicken egg white as a ligand was immobilized onto the functionalized sensor surface and varying concentrations of BB were injected through the SPR channels to evaluate binding kinetics. SPR data were analyzed through multistep software workflow including referencing, baseline correction, alignment, and kinetic fitting. The resulting sensorgrams enabled the determination of key parameters such as association (k
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