ArticleMethods in molecular biology (Clifton, N.J.)2026
Applications of Differential Scanning Fluorimetry and Related Technologies in Characterization of Protein-Ligand Interactions.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Differential scanning fluorimetry (DSF) is an efficient and high-throughput method to analyze protein stability, as well as detect ligand interactions through perturbations of the protein's melting temperature. This method monitors protein unfolding by observing the fluorescence changes of a sample, whether through an environmentally sensitive fluorophore or by intrinsic protein fluorescence, while a temperature gradient is applied. Here, we describe in detail how to develop and optimize DSF assays to identify protein-ligand interactions, while exploring different buffer and additive conditions. Analysis of the data and further applications of the method are also discussed.
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